Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81635
Title: Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli
Authors: Cheng, Ming Soon
Ho, Jia Shin
Lau, Suk Hiang
Chow, Vincent T.K.
Toh, Chee-Seng
Keywords: Biosensor
Virus
Issue Date: 2013
Source: Cheng, M. S., Ho, J. S., Lau, S. H., Chow, V. T., & Toh, C.-S. (2013). Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli. Biosensors and Bioelectronics, 47, 340-344.
Series/Report no.: Biosensors and Bioelectronics
Abstract: We describe an impedimetric microbial sensor for real-time monitoring of the non-lytic M13 bacteriophage infection of Escherichia coli cells using a gold electrode covalently grafted with a monolayer of lipopolysaccharide specific antibody. After infection, damage to the lipopolysaccharide layer on the outer membrane of E. coli causes changes to its surface charge and morphology, resulting in the aggregation of redox probe, Fe(CN)63−/4− at the electrode surface and thereby increases its electron-transfer rate. This consequent decrease of electron-transfer resistance in the presence of bacteriophage can be easily monitored using Faradaic impedance spectroscopy. Non-lytic bacterium–phage interaction which is hardly observable using conventional microscopic methods is detected within 3 h using this impedimetric microbial sensor which demonstrates its excellent performance in terms of analysis time, ease and reduced reliance on labeling steps during in-situ monitoring of the phage infection process.
URI: https://hdl.handle.net/10356/81635
http://hdl.handle.net/10220/40878
ISSN: 0956-5663
DOI: 10.1016/j.bios.2013.03.050
Rights: © 2013 Elsevier.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SPMS Journal Articles

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