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Title: Microfluidic platform for negative enrichment of circulating tumor cells
Authors: Sajay, Bhuvanendran Nair Gourikutty
Chang, Chia-Pin
Ahmad, Hamizah
Khuntontong, Puttachat
Wong, Chee Chung
Wang, Zhiping
Puiu, Poenar Daniel
Soo, Ross
Rahman, Abdur Rub Abdur
Keywords: Circulating tumor cells (CTCs)
Cancer cell isolation
Issue Date: 2014
Source: Sajay, B. N. G., Chang, C.-P., Ahmad, H., Khuntontong, P., Wong, C. C., Wang, Z., et al. (2014). Microfluidic platform for negative enrichment of circulating tumor cells. Biomedical Microdevices, 16(4), 537-548.
Series/Report no.: Biomedical Microdevices
Abstract: Negative enrichment is the preferred approach for tumor cell isolation as it does not rely on biomarker expression. However, size-based negative enrichment methods suffer from well-known recovery/purity trade-off. Non-size based methods have a number of processing steps that lead to compounded cell loss due to extensive sample processing and handling which result in a low recovery efficiency. We present a method that performs negative enrichment in two steps from 2 ml of whole blood in a total assay processing time of 60 min. This negative enrichment method employs upstream immunomagnetic depletion to deplete CD45-positive WBCs followed by a microfabricated filter membrane to perform chemical-free RBC depletion and target cells isolation. Experiments of spiking two cell lines, MCF-7 and NCI-H1975, in the whole blood show an average of >90 % cell recovery over a range of spiked cell numbers. We also successfully recovered circulating tumor cells from 15 cancer patient samples.
ISSN: 1387-2176
DOI: 10.1007/s10544-014-9856-2
Rights: © 2014 Springer Science+Business Media New York.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:EEE Journal Articles

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