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https://hdl.handle.net/10356/81713| Title: | Butelase 1: A Versatile Ligase for Peptide and Protein Macrocyclization | Authors: | Nguyen, Giang Kien Truc Kam, Antony Tam, James Pingkwan Loo, Shining Jansson, Anna Elisabet Pan, Lucy Xin |
Keywords: | Biological Sciences | Issue Date: | 2015 | Source: | Nguyen, G. K. T., Kam, A., Loo, S., Jansson, A. E., Pan, L. X., & Tam, J. P. (2015). Butelase 1: A Versatile Ligase for Peptide and Protein Macrocyclization. Journal of the American Chemical Society, 137(49), 15398-15401. | Series/Report no.: | Journal of the American Chemical Society | Abstract: | Macrocyclization is a valuable tool for drug design and protein engineering. Although various methods have been developed to prepare macrocycles, a general and efficient strategy is needed. Here we report a highly efficient method using butelase 1 to macrocyclize peptides and proteins ranging in sizes from 26 to >200 residues. We achieved cyclizations that are 20,000 times faster than sortase A, the most widely used ligase for protein cyclization. The reactions completed within minutes with up to 95% yields. | URI: | https://hdl.handle.net/10356/81713 http://hdl.handle.net/10220/39652 |
ISSN: | 0002-7863 | DOI: | 10.1021/jacs.5b11014 | Schools: | School of Biological Sciences | Rights: | © 2015 American Chemical Society. This paper was published in Journal of the American Chemical Society and is made available as an electronic reprint (preprint) with permission of American Chemical Society. The published version is available at: [http://dx.doi.org/10.1021/jacs.5b11014]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
| Appears in Collections: | SBS Journal Articles |
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|---|---|---|---|---|
| Butelase 1 A Versatile Ligase for Peptide and Protein Macrocyclization.pdf | 2.29 MB | Adobe PDF |  View/Open |
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