Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81713
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dc.contributor.authorNguyen, Giang Kien Trucen
dc.contributor.authorKam, Antonyen
dc.contributor.authorTam, James Pingkwanen
dc.contributor.authorLoo, Shiningen
dc.contributor.authorJansson, Anna Elisabeten
dc.contributor.authorPan, Lucy Xinen
dc.date.accessioned2016-01-11T05:27:39Zen
dc.date.accessioned2019-12-06T14:36:42Z-
dc.date.available2016-01-11T05:27:39Zen
dc.date.available2019-12-06T14:36:42Z-
dc.date.issued2015en
dc.identifier.citationNguyen, G. K. T., Kam, A., Loo, S., Jansson, A. E., Pan, L. X., & Tam, J. P. (2015). Butelase 1: A Versatile Ligase for Peptide and Protein Macrocyclization. Journal of the American Chemical Society, 137(49), 15398-15401.en
dc.identifier.issn0002-7863en
dc.identifier.urihttps://hdl.handle.net/10356/81713-
dc.description.abstractMacrocyclization is a valuable tool for drug design and protein engineering. Although various methods have been developed to prepare macrocycles, a general and efficient strategy is needed. Here we report a highly efficient method using butelase 1 to macrocyclize peptides and proteins ranging in sizes from 26 to >200 residues. We achieved cyclizations that are 20,000 times faster than sortase A, the most widely used ligase for protein cyclization. The reactions completed within minutes with up to 95% yields.en
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en
dc.format.extent4 p.en
dc.language.isoenen
dc.relation.ispartofseriesJournal of the American Chemical Societyen
dc.rights© 2015 American Chemical Society. This paper was published in Journal of the American Chemical Society and is made available as an electronic reprint (preprint) with permission of American Chemical Society. The published version is available at: [http://dx.doi.org/10.1021/jacs.5b11014]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.subjectBiological Sciencesen
dc.titleButelase 1: A Versatile Ligase for Peptide and Protein Macrocyclizationen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.1021/jacs.5b11014en
dc.description.versionPublished versionen
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