Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81779
Title: Topographical effects on fiber-mediated microRNA delivery to control oligodendroglial precursor cells development
Authors: Diao, Hua Jia
Low, Wei Ching
Lu, Q. Richard
Chew, Sing Yian
Keywords: Nanofibers
Electrospinning
Issue Date: 2015
Source: Diao, H. J., Low, W. C., Lu, Q. R., & Chew, S. Y. (2015). Topographical effects on fiber-mediated microRNA delivery to control oligodendroglial precursor cells development. Biomaterials, 70, 105-114.
Series/Report no.: Biomaterials
Abstract: Effective remyelination in the central nervous system (CNS) facilitates the reversal of disability in patients with demyelinating diseases such as multiple sclerosis. Unfortunately until now, effective strategies of controlling oligodendrocyte (OL) differentiation and maturation remain limited. It is well known that topographical and biochemical signals play crucial roles in modulating cell fate commitment. Therefore, in this study, we explored the combined effects of scaffold topography and sustained gene silencing on oligodendroglial precursor cell (OPC) development. Specifically, microRNAs (miRs) were incorporated onto electrospun polycaprolactone (PCL) fiber scaffolds with different fiber diameters and orientations. Regardless of fiber diameter and orientation, efficient knockdown of differentiation inhibitory factors were achieved by either topography alone (up to 70%) or fibers integrated with miR-219 and miR-338 (up to 80%, p < 0.05). Small fiber promoted OPC differentiation by inducing more RIP+ cells (p < 0.05) while large fiber promoted OL maturation by inducing more MBP+ cells (p < 0.05). Random fiber enhanced more RIP+ cells than aligned fibers (p < 0.05), regardless of fiber diameter. Upon miR-219/miR-338 incorporation, 2 μm aligned fibers supported the most MBP+ cells (∼17%). These findings indicated that the coupling of substrate topographic cues with efficient gene silencing by sustained microRNA delivery is a promising way for directing OPC maturation in neural tissue engineering and controlling remyelination in the CNS.
URI: https://hdl.handle.net/10356/81779
http://hdl.handle.net/10220/40992
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2015.08.029
Schools: School of Chemical and Biomedical Engineering 
Lee Kong Chian School of Medicine (LKCMedicine) 
Rights: © 2015 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Biomaterials, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.biomaterials.2015.08.029].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SCBE Journal Articles

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