Please use this identifier to cite or link to this item:
|Title:||The nuage mediates retrotransposon silencing in mouse primordial ovarian follicles||Authors:||Lim, Ai Khim
Chew, Ting Gang
Tan, Chin Wee Godwin
Shue, Yan Ting
Matzuk, Martin M.
Messerschmidt, Daniel M.
Knowles, Barbara B.
|Issue Date:||2013||Source:||Lim, A. K., Lorthongpanich, C., Chew, T. G., Tan, C. W. G., Shue, Y. T., Balu, S., et al. (2013). The nuage mediates retrotransposon silencing in mouse primordial ovarian follicles. Development, 140, 3819-3825.||Series/Report no.:||Development||Abstract:||Mobilization of endogenous retrotransposons can destabilize the genome, an imminent danger during epigenetic reprogramming of cells in the germline. The P-element-induced wimpy testis (PIWI)-interacting RNA (piRNA) pathway is known to silence retrotransposons in the mouse testes. Several piRNA pathway components localize to the unique, germline structure known as the nuage. In this study, we surveyed mouse ovaries and found, for the first time, transient appearance of nuage-like structures in oocytes of primordial follicles. Mouse vasa homolog (MVH), Piwi-like 2 (PIWIL2/MILI) and tudor domain-containing 9 (TDRD9) are present in these structures, whereas aggregates of germ cell protein with ankyrin repeats, sterile alpha motif and leucine zipper (GASZ) localize separately in the cytoplasm. Retrotransposons are silenced in primordial ovarian follicles, and de-repressed upon reduction of piRNA expression in Mvh, Mili or Gasz mutants. However, these null-mutant females, unlike their male counterparts, are fertile, uncoupling retrotransposon activation from sterility.||URI:||https://hdl.handle.net/10356/81827
|ISSN:||0950-1991||DOI:||10.1242/dev.099184||Schools:||School of Biological Sciences||Rights:||© 2013 The Authors (published by The Company of Biologists Ltd.)||Fulltext Permission:||none||Fulltext Availability:||No Fulltext|
|Appears in Collections:||SBS Journal Articles|
Updated on Sep 24, 2023
Web of ScienceTM
Updated on Sep 20, 2023
Updated on Sep 26, 2023
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.