Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81833
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dc.contributor.authorQian, Xinleien
dc.contributor.authorHamid, Fursham M.en
dc.contributor.authorEl Sahili, Abbasen
dc.contributor.authorDarwis, Dina Amalliaen
dc.contributor.authorWong, Yee Hwaen
dc.contributor.authorBhushan, Shashien
dc.contributor.authorMakeyev, Eugene V.en
dc.contributor.authorLescar, Julienen
dc.date.accessioned2016-07-27T02:30:13Zen
dc.date.accessioned2019-12-06T14:41:09Z-
dc.date.available2016-07-27T02:30:13Zen
dc.date.available2019-12-06T14:41:09Z-
dc.date.issued2016en
dc.identifier.citationQian, X., Hamid, F. M., El Sahili, A., Darwis, D. A., Wong, Y. H., Bhushan, S., et al. (2016). Functional Evolution in Orthologous Cell-encoded RNA-dependent RNA Polymerases. Journal of Biological Chemistry, 291(17), 9295-9309.en
dc.identifier.issn0021-9258en
dc.identifier.urihttps://hdl.handle.net/10356/81833-
dc.description.abstractMany eukaryotic organisms encode more than one RNA-dependent RNA polymerase (RdRP) that likely emerged as a result of gene duplication. Such RdRP paralogs often participate in distinct RNA silencing pathways and show characteristic repertoires of enzymatic activities in vitro. However, to what extent members of individual paralogous groups can undergo functional changes during speciation remains an open question. We show that orthologs of QDE-1, an RdRP component of the quelling pathway in Neurospora crassa, have rapidly diverged in evolution at the amino acid sequence level. Analyses of purified QDE-1 polymerases from N. crassa (QDE-1Ncr) and related fungi, Thielavia terrestris (QDE-1Tte) and Myceliophthora thermophila (QDE-1Mth), show that all three enzymes can synthesize RNA but the precise modes of their action differ considerably. Unlike their QDE-1Ncr counterpart favoring processive RNA synthesis, QDE-1Tte and QDE-1Mth produce predominantly short RNA copies via primer-independent initiation. Surprisingly, a 3.19 Å-resolution crystal structure of QDE-1Tte reveals a quasi-symmetric dimer similar to QDE-1Ncr. Further electron microscopy analyses confirm that QDE-1Tte occurs as a dimer in solution and retains this status upon interaction with a template. We conclude that divergence of orthologous RdRPs can result in functional innovation while retaining overall protein fold and quaternary structure.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en
dc.format.extent31 p.en
dc.language.isoenen
dc.relation.ispartofseriesJournal of Biological Chemistryen
dc.rights© 2016 The American Society for Biochemistry and Molecular Biology. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Biological Chemistry, The American Society for Biochemistry and Molecular Biology. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1074/jbc.M115.685933].en
dc.subjectRNA-dependent RNA polymeraseen
dc.subjectsmall RNAsen
dc.titleFunctional Evolution in Orthologous Cell-encoded RNA-dependent RNA Polymerasesen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.1074/jbc.M115.685933en
dc.description.versionAccepted versionen
dc.identifier.pmid26907693-
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