Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81846
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dc.contributor.authorLiu, Xiaohuen
dc.contributor.authorWang, Yien
dc.contributor.authorChen, Pengen
dc.contributor.authorWang, Yusongen
dc.contributor.authorZhang, Jinlingen
dc.contributor.authorAili, Danielen
dc.contributor.authorLiedberg, Boen
dc.date.accessioned2016-01-22T01:42:34Zen
dc.date.accessioned2019-12-06T14:41:27Z-
dc.date.available2016-01-22T01:42:34Zen
dc.date.available2019-12-06T14:41:27Z-
dc.date.issued2014en
dc.identifier.citationLiu, X., Wang, Y., Chen, P., Wang, Y., Zhang, J., Aili, D., et al. (2014). Biofunctionalized Gold Nanoparticles for Colorimetric Sensing of Botulinum Neurotoxin A Light Chain. Analytical Chemistry, 86(5), 2345-2352.en
dc.identifier.issn0003-2700en
dc.identifier.urihttps://hdl.handle.net/10356/81846-
dc.identifier.urihttp://hdl.handle.net/10220/39747en
dc.description.abstractBotulinum neurotoxin is considered as one of the most toxic food-borne substances and is a potential bioweapon accessible to terrorists. The development of an accurate, convenient, and rapid assay for botulinum neurotoxins is therefore highly desirable for addressing biosafety concerns. Herein, novel biotinylated peptide substrates designed to mimic synaptosomal-associated protein 25 (SNAP-25) are utilized in gold nanoparticle-based assays for colorimetric detection of botulinum neurotoxin serotype A light chain (BoLcA). In these proteolytic assays, biotinylated peptides serve as triggers for the aggregation of gold nanoparticles, while the cleavage of these peptides by BoLcA prevents nanoparticle aggregation. Two different assay strategies are described, demonstrating limits of detection ranging from 5 to 0.1 nM of BoLcA with an overall assay time of 4 h. These hybrid enzyme-responsive nanomaterials provide rapid and sensitive detection for one of the most toxic substances known to man.en
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en
dc.format.extent24 p.en
dc.language.isoenen
dc.relation.ispartofseriesAnalytical Chemistryen
dc.rights© 2014 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical Chemistry, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/ac402626g].en
dc.subjectBiological weaponsen
dc.subjectAssaysen
dc.titleBiofunctionalized Gold Nanoparticles for Colorimetric Sensing of Botulinum Neurotoxin A Light Chainen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science and Engineeringen
dc.contributor.researchCentre for Biomimetic Sensor Scienceen
dc.identifier.doihttp://dx.doi.org/10.1021/ac402626gen
dc.description.versionAccepted versionen
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Appears in Collections:MSE Journal Articles
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