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Title: De Novo Reconstruction of Adipose Tissue Transcriptomes Reveals Long Non-coding RNA Regulators of Brown Adipocyte Development
Authors: Alvarez-Dominguez, Juan R.
Bai, Zhiqiang
Xu, Dan
Yuan, Bingbing
Lo, Kinyui Alice
Yoon, Myeong Jin
Lim, Yen Ching
Knoll, Marko
Slavov, Nikolai
Chen, Shuai
Chen, Peng
Lodish, Harvey F.
Sun, Lei
Keywords: Cell Metabolism
Issue Date: 2015
Source: Alvarez-Dominguez, J. R., Bai, Z., Xu, D., Yuan, B., Lo, K. A., Yoon, M. J., et al. (2015). De Novo Reconstruction of Adipose Tissue Transcriptomes Reveals Long Non-coding RNA Regulators of Brown Adipocyte Development. Cell Metabolism, 21(5), 764-776.
Series/Report no.: Cell Metabolism
Abstract: Brown adipose tissue (BAT) protects against obesity by promoting energy expenditure via uncoupled respiration. To uncover BAT-specific long non-coding RNAs (lncRNAs), we used RNA-seq to reconstruct de novo transcriptomes of mouse brown, inguinal white, and epididymal white fat and identified ∼1,500 lncRNAs, including 127 BAT-restricted loci induced during differentiation and often targeted by key regulators PPARγ, C/EBPα, and C/EBPβ. One of them, lnc-BATE1, is required for establishment and maintenance of BAT identity and thermogenic capacity. lnc-BATE1 inhibition impairs concurrent activation of brown fat and repression of white fat genes and is partially rescued by exogenous lnc-BATE1 with mutated siRNA-targeting sites, demonstrating a function in trans. We show that lnc-BATE1 binds heterogeneous nuclear ribonucleoprotein U and that both are required for brown adipogenesis. Our work provides an annotated catalog for the study of fat depot-selective lncRNAs and establishes lnc-BATE1 as a regulator of BAT development and physiology.
ISSN: 1550-4131
DOI: 10.1016/j.cmet.2015.04.003
Schools: School of Chemical and Biomedical Engineering 
Rights: © 2015 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Cell Metabolism, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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