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Title: Organic Nanoprobe Cocktails for Multilocal and Multicolor Fluorescence Imaging of Reactive Oxygen Species
Authors: Yin, Chao
Zhu, Houjuan
Xie, Chen
Zhang, Lei
Chen, Peng
Fan, Quli
Huang, Wei
Pu, Kanyi
Keywords: Activatable probes
Fluorescence imaging
Issue Date: 2017
Source: Yin, C., Zhu, H., Xie, C., Zhang, L., Chen P., Fan, Q., et al. (2017). Organic Nanoprobe Cocktails for Multilocal and Multicolor Fluorescence Imaging of Reactive Oxygen Species. Advanced Functional Materials, 27(23), 1700493-.
Series/Report no.: Advanced Functional Materials
Abstract: Hypochlorite (ClO−) as a highly reactive oxygen species not only acts as a powerful “guarder” in innate host defense but also regulates inflammation-related pathological conditions. Despite the availability of fluorescence probes for detection of ClO− in cells, most of them can only detect ClO− in single cellular organelle, limiting the capability to fully elucidate the synergistic effect of different organelles on the generation of ClO−. This study proposes a nanoprobe cocktail approach for multicolor and multiorganelle imaging of ClO− in cells. Two semiconducting oligomers with different π-conjugation length are synthesized, both of which contain phenothiazine to specifically react with ClO− but show different fluorescent color responses. These sensing components are self-assembled into the nanoprobes with the ability to target cellular lysosome and mitochondria, respectively. The mixture of these nanoprobes forms a nano-cocktail that allows for simultaneous imaging of elevated level of ClO− in lysosome and mitochondria according to fluorescence color variations under selective excitation of each nanoprobe. Thus, this study provides a general concept to design probe cocktails for multilocal and multicolor imaging.
ISSN: 1616-301X
DOI: 10.1002/adfm.201700493
Schools: School of Chemical and Biomedical Engineering 
Rights: © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the author created version of a work that has been peer reviewed and accepted for publication by Advanced Functional Materials, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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