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Title: Insight into membrane selectivity of linear and branched polyethylenimines and their potential as biocides for advanced wound dressings
Authors: Fox, Stephen John
Fazil, Mobashar Hussain Urf Turabe
Dhand, Chetna
Venkatesh, Mayandi
Goh, Eunice Tze Leng
Harini, Sriram
Eugene, Christo
Lim, Rayne Rui
Ramakrishna, Seeram
Chaurasia, Shyam Sunder
Beuerman, Roger W.
Verma, Chandra Shekhar
Verma, Navin Kumar
Loh, Xian Jun
Lakshminarayanan, Rajamani
Keywords: Molecular dynamics
Membrane selectivity
Issue Date: 2016
Source: Fox, S. J., Fazil, M. H. U. T., Dhand, C., Venkatesh, M., Goh, E. T. L., Harini, S., et al. (2016). Insight into membrane selectivity of linear and branched polyethylenimines and their potential as biocides for advanced wound dressings. Acta Biomaterialia, 37, 155-164.
Series/Report no.: Acta Biomaterialia
Abstract: We report here structure-property relationship between linear and branched polyethylene imines by examining their antimicrobial activities against wide range of pathogens. Both the polymers target the cytoplasmic membrane of bacteria and yeasts, eliciting rapid microbicidal properties. Using multiscale molecular dynamic simulations, we showed that, in both fully or partially protonated forms LPEI discriminates between mammalian and bacterial model membranes whereas BPEI lacks selectivity for both the model membranes. Simulation results suggest that LPEI forms weak complex with the zwitterionic lipids whereas the side chain amino groups of BPEI sequester the zwitterionic lipids by forming tight complex. Consistent with these observations, label-free cell impedance measurements, cell viability assays and high content analysis indicate that BPEI is cytotoxic to human epithelial and fibroblasts cells. Crosslinking of BPEI onto electrospun gelatin mats attenuate the cytotoxicity for fibroblasts while retaining the antimicrobial activity against Gram-positive and yeasts strains. PEI crosslinked gelatin mats elicit bactericidal activity by contact-mediated killing and durable to leaching for 7days. The potent antimicrobial activity combined with enhanced selectivity of the crosslinked ES gelatin mats would expand the arsenel of biocides in the management of superficial skin infections. The contact-mediated microbicidal properties may avert antimicrobial resistance and expand the diversity of applications to prevent microbial contamination.
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2016.04.015
Rights: © 2016 Acta Materialia Inc. (published by Elsevier Ltd.). This is the author created version of a work that has been peer reviewed and accepted for publication in Acta Biomaterialia, published by Elsevier Ltd. on behalf of Acta Materialia Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document.  The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
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