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Title: In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology
Authors: Chew, Su Chuen
Kjelleberg, Staffan
Yang, Liang
Rice, Scott A.
Keywords: Matrix
Issue 106
Particle-tracking microrheology
Issue Date: 2015
Source: Chew, S.C., Rice, S.A., Kjelleberg, S., & Yang, L. (2015) In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology. Journal of Visualized Experiments, (106), e53093-.
Series/Report no.: Journal of Visualized Experiments
Abstract: Bacterial cells are able to form surface-attached biofilm communities known as biofilms by encasing themselves in extracellular polymeric substances (EPS). The EPS serves as a physical and protective scaffold that houses the bacterial cells and consists of a variety of materials that includes proteins, exopolysaccharides and DNA. The composition of the EPS may change, which remodels the mechanic properties of the biofilm to further develop or support alternative biofilm structures, such as streamers, as a response to environmental cues. Despite this, there are little quantitative descriptions on how EPS components contribute to the mechanical properties and function of biofilms. Rheology, the study of the flow of matter, is of particular relevance to biofilms as many biofilms grow in flow conditions and are constantly exposed to shear stress. It also provides measurement and insight on the spreading of the biofilm on a surface. Here, particle-tracking microrheology is used to examine the viscoelasticity and effective crosslinking roles of different matrix components in various parts of the biofilm during development. This approach allows researchers to measure mechanic properties of biofilms at the micro-scale, which might provide useful information for controlling and engineering biofilms.
DOI: 10.3791/53093
Rights: © 2015 Journal of Visualized Experiments. This paper was published in Journal of Visualized Experiments and is made available as an electronic reprint (preprint) with permission of Journal of Visualized Experiments. The published version is available at: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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