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Title: Native chemical ubiquitination using a genetically incorporated azidonorleucine
Authors: Yang, Renliang
Bi, Xiaobao
Li, Fupeng
Cao, Yuan
Liu, Chuan-Fa
Keywords: Biological Sciences
Issue Date: 2014
Source: Yang, R., Bi, X., Li, F., Cao, Y., & Liu, C.-F. (2014). Native chemical ubiquitination using a genetically incorporated azidonorleucine. Chemical Communications, 50(59), 7971-7974.
Series/Report no.: Chemical Communications
Abstract: A robust chemical ubiquitination method was developed. The method employed a genetically incorporated azidonorleucine as an orthogonal lysine precursor for the installation of a Gly residue bearing an Nα-auxiliary which mediated the ligation between ubiquitin(1–75)-thioester and the target protein. To demonstrate our methodology, a model protein, K48-linked diubiquitin, was synthesized with an overall yield of 35%.
ISSN: 1359-7345
DOI: 10.1039/c4cc03721a
Schools: School of Biological Sciences 
Rights: © 2014 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Chemical Communications, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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