Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/82098
Title: Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens
Authors: Ye, Fuzhou
Wang, Chao
Fu, Qinqin
Zhang, Lian-hui
Gao, Yong-Gui
Keywords: SghR
SghA
Issue Date: 2015
Source: Ye, F., Wang, C., Fu, Q., Zhang, L., & Gao, Y. (2015). Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens. Acta Crystallographica Section F: Structural Biology Communications, 71(9), 1139-1145.
Series/Report no.: Acta Crystallographica Section F: Structural Biology Communications
Abstract: Two proteins, SghA and SghR, which were recently identified and characterized as novel bacterial virulence factors regulating the infection of plant hosts by Agrobacterium, were cloned, overexpressed and purified with high yield. Both SghA and SghR form dimers in solution. The purified SghA and SghR were crystallized and the crystals diffracted to 1.9 and 2.1 Å resolution, respectively. Data were collected and processed, and the crystallographic parameters were within acceptable ranges. These results will help in the determination of their structures in order to uncover the molecular mechanism of how these two proteins together control the release of plant defence signals against agrobacteria during pathogen-host interaction.
URI: https://hdl.handle.net/10356/82098
http://hdl.handle.net/10220/41125
ISSN: 2053-230X
DOI: 10.1107/S2053230X15012881
Rights: © 2015 International Union of Crystallography (IUCr). This paper was published in Acta Crystallographica Section F: Structural Biology Communications and is made available as an electronic reprint (preprint) with permission of International Union of Crystallography (IUCr). The published version is available at: [http://dx.doi.org/10.1107/S2053230X15012881]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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