Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/82159
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dc.contributor.authorYusufi, Faraaz N. K.en
dc.contributor.authorYang, Yuanshengen
dc.contributor.authorLee, Dong-Yupen
dc.contributor.authorChung, Bevan Kai-Shengen
dc.contributor.authorMariatien
dc.date.accessioned2016-08-05T06:51:56Zen
dc.date.accessioned2019-12-06T14:47:44Z-
dc.date.available2016-08-05T06:51:56Zen
dc.date.available2019-12-06T14:47:44Z-
dc.date.issued2013en
dc.identifier.citationChung, B. K.-S., Yusufi, F. N. K., Mariati, Yang, Y., & Lee, D.-Y. (2013). Enhanced expression of codon optimized interferon gamma in CHO cells. Journal of Biotechnology, 167(3), 326-333.en
dc.identifier.issn0168-1656en
dc.identifier.urihttps://hdl.handle.net/10356/82159-
dc.description.abstractThe human interferon-gamma (IFN-γ) is a potential drug candidate for treating various diseases due to its immunomodulatory properties. The efficient production of this protein can be achieved through a popular industrial host, Chinese hamster ovary (CHO) cells. However, recombinant expression of foreign proteins is typically suboptimal possibly due to the usage of non-native codon patterns within the coding sequence. Therefore, we demonstrated the application of a recently developed codon optimization approach to design synthetic IFN-γ coding sequences for enhanced heterologous expression in CHO cells. For codon optimization, earlier studies suggested to establish the target usage distribution pattern in terms of selected design parameters such as individual codon usage (ICU) and codon context (CC), mainly based on the host's highly expressed genes. However, our RNA-Seq based transcriptome profiling indicated that the ICU and CC distribution patterns of different gene expression classes in CHO cell are relatively similar, unlike other microbial expression hosts, Escherichia coli and Saccharomyces cerevisiae. This finding was further corroborated through the in vivo expression of various ICU and CC optimized IFN-γ in CHO cells. Interestingly, the CC-optimized genes exhibited at least 13-fold increase in expression level compared to the wild-type IFN-γ while a maximum of 10-fold increase was observed for the ICU-optimized genes. Although design criteria based on individual codons, such as ICU, have been widely used for gene optimization, our experimental results suggested that codon context is relatively more effective parameter for improving recombinant IFN-γ expression in CHO cells.en
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en
dc.format.extent8 p.en
dc.language.isoenen
dc.relation.ispartofseriesJournal of Biotechnologyen
dc.rights© 2013 Elsevier B.V.en
dc.subjectChinese hamster ovary cellsen
dc.subjectCodon optimizationen
dc.titleEnhanced expression of codon optimized interferon gamma in CHO cellsen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.identifier.doi10.1016/j.jbiotec.2013.07.011en
item.grantfulltextnone-
item.fulltextNo Fulltext-
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