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Title: A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes
Authors: Tie, Hieng Chiong
Mahajan, Divyanshu
Chen, Bing
Cheng, Li
VanDongen, Antonius M. J.
Lu, Lei
Keywords: Membrane Trafficking
Issue Date: 2016
Source: Tie, H. C., Mahajan, D., Chen, B., Cheng, L., VanDongen, A. M. J., & Lu, L. (2016). A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes. Molecular Biology of the Cell, 27(5), 848-861.
Series/Report no.: Molecular Biology of the Cell
Abstract: Cellular functions of the Golgi are determined by the unique distribution of its resident proteins. Currently, electron microscopy is required for the localization of a Golgi protein at the sub-Golgi level. We developed a quantitative sub-Golgi localization method based on centers of fluorescence masses of nocodazole-induced Golgi ministacks under conventional optical microscopy. Our method is rapid, convenient, and quantitative, and it yields a practical localization resolution of ∼30 nm. The method was validated by the previous electron microscopy data. We quantitatively studied the intra-Golgi trafficking of synchronized secretory membrane cargoes and directly demonstrated the cisternal progression of cargoes from the cis- to the trans-Golgi. Our data suggest that the constitutive efflux of secretory cargoes could be restricted at the Golgi stack, and the entry of the trans-Golgi network in secretory pathway could be signal dependent.
ISSN: 1059-1524
DOI: 10.1091/mbc.E15-09-0664
Schools: School of Biological Sciences 
Rights: © 2016 Tie et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License ( “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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