Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/82297
Title: Phosphatase POPX2 Exhibits Dual Regulatory Functions in Cancer Metastasis
Authors: Zhang, Songjing
Weng, Ting
Cheruba, Elsie
Guo, Tiannan
Chan, Hei
Sze, Siu Kwan
Koh, Cheng-Gee
Keywords: SILAC mass spectrometry
Secretome
Issue Date: 2016
Source: Zhang, S., Weng, T., Cheruba, E., Guo, T., Chan, H., Sze, S. K., et al. (2017). Phosphatase POPX2 Exhibits Dual Regulatory Functions in Cancer Metastasis. Journal of Proteome Research, 16(2), 698-711.
Series/Report no.: Journal of Proteome Research
Abstract: Cancer metastasis is a complex mechanism involving multiple processes. Previously, our integrative proteome, transcriptome, and phosphoproteome study reported that the levels of serine/threonine phosphatase POPX2 were positively correlated with cancer cell motility through modulating MAPK signaling. Surprisingly, here we found that POPX2 knockdown cells induced more numerous and larger tumor nodules in lungs in longer term animal studies. Interestingly, our analysis of DNA microarray data from cancer patient samples that are available in public databases shows that low POPX2 expression is linked to distant metastasis and poor survival rate. These observations suggest that lower levels of POPX2 may favor tumor progression in later stages of metastasis. We hypothesize that POPX2 may do so by modulation of angiogenesis. Secretome analysis of POPX2-knockdown MDA-MB-231 cells using LC–MS/MS-based SILAC quantitative proteomics and cytokine array show that silencing of POPX2 leads to increased secretion of exosomes, which may, in turn, induce multiple pro-angiogenic cytokines. This study, combined with our previous findings, suggests that a single ubiquitously expressed phosphatase POPX2 influences cancer metastasis via modulating multiple biological processes including MAPK signaling and exosome cytokine secretion.
URI: https://hdl.handle.net/10356/82297
http://hdl.handle.net/10220/43514
ISSN: 1535-3893
DOI: 10.1021/acs.jproteome.6b00748
Schools: School of Biological Sciences 
Rights: © 2016 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Proteome Research, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acs.jproteome.6b00748].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

Files in This Item:
File Description SizeFormat 
Phosphatase POPX2 exhibits dual regulatory functions in cancer metastasis.pdf5.46 MBAdobe PDFThumbnail
View/Open

SCOPUSTM   
Citations 20

12
Updated on Jun 15, 2024

Web of ScienceTM
Citations 20

10
Updated on Oct 28, 2023

Page view(s) 50

459
Updated on Jun 17, 2024

Download(s) 20

253
Updated on Jun 17, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.