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Title: Talin1 methylation is required for neutrophil infiltration and lipopolysaccharide-induced lethality
Authors: Lim, Thomas Jun Feng
Su, I-hsin
Keywords: Neutrophil
Science::Biological sciences
Issue Date: 2018
Source: Lim, T. J. F., & Su, I.-H. (2018). Talin1 methylation is required for neutrophil infiltration and lipopolysaccharide-induced lethality. Journal of Immunology, 201(12), 3651-3661. doi:10.4049/jimmunol.1800567
Series/Report no.: Journal of Immunology
Abstract: Talin1, a well-established integrin coactivator, is critical for the transmigration of neutrophils across the vascular endothelium into various organs and the peritoneal cavity during inflammation. Several posttranslational modifications of talin1 have been proposed to play a role in this process. In this study, we show that trimethylation of talin1 at Lys2454 by cytosolic Ezh2 is substantially increased in murine peritoneal neutrophils upon induction of peritonitis. By reconstituting talin1-deficient mouse myeloid cells with wild-type, methyl-mimicking, or unmethylatable talin1 variants, we demonstrate that methylation of talin1 at Lys2454 is important for integrin-dependent neutrophil infiltration into the peritoneal cavity. Furthermore, we show that treatment with an Ezh2 inhibitor or reconstitution of talin1-deficient myeloid cells with unmethylatable talin1 significantly reduces the number of organ-infiltrating neutrophils and protects mice from LPS-induced mortality.
ISSN: 0022-1767
DOI: 10.4049/jimmunol.1800567
DOI (Related Dataset):
Schools: School of Social Sciences 
Rights: © 2018 The American Association of Immunologists, Inc. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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