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Title: Embryonic Stem Cells Exhibit mRNA Isoform Specific Translational Regulation
Authors: Wong, Queenie Wing-Lei
Vaz, Candida
Lee, Qian Yi
Zhao, Tian Yun
Luo, Raymond
Archer, Stuart K.
Preiss, Thomas
Tanavde, Vivek
Vardy, Leah Anne
Keywords: NPC
Neural Precursor Cell
Embryonic Stem Cell
Issue Date: 2016
Source: Wong, Q. W.-L., Vaz, C., Lee, Q. Y., Zhao, T. Y., Luo, R., Archer, S. K., et al. (2016). Embryonic Stem Cells Exhibit mRNA Isoform Specific Translational Regulation. PLoS ONE, 11(1), e0143235-.
Series/Report no.: PLoS ONE
Abstract: The presence of multiple variants for many mRNAs is a major contributor to protein diversity. The processing of these variants is tightly controlled in a cell-type specific manner and has a significant impact on gene expression control. Here we investigate the differential translation rates of individual mRNA variants in embryonic stem cells (ESCs) and in ESC derived Neural Precursor Cells (NPCs) using polysome profiling coupled to RNA sequencing. We show that there are a significant number of detectable mRNA variants in ESCs and NPCs and that many of them show variant specific translation rates. This is correlated with differences in the UTRs of the variants with the 5’UTR playing a predominant role. We suggest that mRNA variants that contain alternate UTRs are under different post-transcriptional controls. This is likely due to the presence or absence of miRNA and protein binding sites that regulate translation rate. This highlights the importance of addressing translation rate when using mRNA levels as a read out of protein abundance. Additional analysis shows that many annotated non-coding mRNAs are present on the polysome fractions in ESCs and NPCs. We believe that the use of polysome fractionation coupled to RNA sequencing is a useful method for analysis of the translation state of many different RNAs in the cell.
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0143235
Schools: School of Biological Sciences 
Rights: © 2016 Wong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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