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Title: Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system
Authors: Xiao, Keke
Guo, Chenghong
Zhou, Yan
Maspolim, Yogananda
Ng, Wun-Jern
Keywords: Two-stage anaerobic system
Acetic acid inhibition
Hydrogenotrophic methanogens
Biomass activity
Issue Date: 2015
Source: Xiao, K., Guo, C., Zhou, Y., Maspolim, Y., & Ng, W.-J. (2016). Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system. Chemosphere, 144, 1498-1504.
Series/Report no.: Chemosphere
Abstract: This study reports on biomass tolerance towards high concentrations of acetic acid (HAc) within the system. Biomass from the second stage of a two-stage anaerobic sludge digestion system was used for this study. Microbial community analysis by 454 pyrosequencing highlighted hydrogenotrophic Methanomicrobiales was the predominant archaeal population in the second stage (>99% of the total archaeal community). Second stage biomass degraded HAc up to 4200 mg HAc L-1 without observable lag phase. However, at HAc-shock loading of 7400 mg HAc L-1, it showed a one day lag phase associated with decreased biomass activity. After stepwise HAc-acclimation over 27 d, the biomass degraded HAc of up to 8200 mg HAc L-1 without observable lag phase. The dominance of Methanomicrobiales had remained unchanged in proportion - while the total archaeal population increased during acclimation. This study showed stepwise acclimation could be an approach to accommodate HAc accumulation and hence higher concentrations resulting from an enhanced first stage. Keywords: Acclimation; Acetic acid inhibition; Biomass activity; Hydrogenotrophic methanogens; Pyrosequencing; Two-stage anaerobic system
ISSN: 0045-6535
DOI: 10.1016/j.chemosphere.2015.10.035
Schools: School of Civil and Environmental Engineering 
Research Centres: Nanyang Environment and Water Research Institute 
Rights: © 2015 Elsevier Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Chemosphere, Elsevier Ltd. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:CEE Journal Articles
NEWRI Journal Articles

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