Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/82960
Title: Lipopolysaccharide-affinity copolymer senses the rapid motility of swarmer bacteria to trigger antimicrobial drug release
Authors: Yeow, Edwin Kok Lee
Lu, Shengtao
Bi, Wuguo
Du, Quanchao
Sinha, Sheetal
Wu, Xiangyang
Subrata, Arnold
Bhattacharjya, Surajit
Xing, Bengang
Keywords: DRNTU::Science::Chemistry::Biochemistry
Swarmer Bacteria
Drug Release
Issue Date: 2018
Source: Lu, S., Bi, W., Du, Q., Sinha, S., Wu, X., Subrata, A., . . . Yeow, E. K. L. (2018). Lipopolysaccharide-affinity copolymer senses the rapid motility of swarmer bacteria to trigger antimicrobial drug release. Nature Communications, 9(1), 4277-. doi:10.1038/s41467-018-06729-6
Series/Report no.: Nature Communications
Abstract: An intelligent drug release system that is triggered into action upon sensing the motion of swarmer P. mirabilis is introduced. The rational design of the drug release system focuses on a pNIPAAm-co-pAEMA copolymer that prevents drug leakage in a tobramycin-loaded mesoporous silica particle by covering its surface via electrostatic attraction. The copolymer chains are also conjugated to peptide ligands YVLWKRKRKFCFI-NH2 that display affinity to Gram-negative bacteria. When swarmer P. mirabilis cells approach and come in contact with the particle, the copolymer-YVLWKRKRKFCFI-NH2 binds to the lipopolysaccharides on the outer membrane of motile P. mirabilis and are stripped off the particle surface when the cells move away; hence releasing tobramycin into the swarmer colony and inhibiting its expansion. The release mechanism is termed Motion-Induced Mechanical Stripping (MIMS). For swarmer B. subtilis, the removal of copolymers from particle surfaces via MIMS is not apparent due to poor adherence between bacteria and copolymer-YVLWKRKRKFCFI-NH2 system.
URI: https://hdl.handle.net/10356/82960
http://hdl.handle.net/10220/47529
DOI: 10.1038/s41467-018-06729-6
Rights: © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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