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Title: Structure of the NS2B-NS3 protease from Zika virus after self-cleavage
Authors: Phoo, Wint Wint
Li, Yan
Zhang, Zhenzhen
Lee, Michelle Yueqi
Loh, Ying Ru
Tan, Yaw Bia
Ng, Elizabeth Yihui
Lescar, Julien
Kang, CongBao
Luo, Dahai
Keywords: Zika virus
Issue Date: 2016
Source: Phoo, W. W., Li, Y., Zhang, Z., Lee, M. Y., Loh, Y. R., Tan, Y. B., et al. (2016). Structure of the NS2B-NS3 protease from Zika virus after self-cleavage. Nature Communications, 7, 13410-.
Series/Report no.: Nature Communications
Abstract: The recent outbreak of Zika virus (ZIKV) infections in the Americas represents a serious threat to the global public health. The viral protease that processes viral polyproteins during infection appears as an attractive drug target. Here we report a crystal structure at 1.84 Å resolution of ZIKV non-structural protein NS2B-NS3 protease with the last four amino acids of the NS2B cofactor bound at the NS3 active site. This structure represents a post-proteolysis state of the enzyme during viral polyprotein processing and provides insights into peptide substrate recognition by the protease. Nuclear magnetic resonance (NMR) studies and protease activity assays unravel the protein dynamics upon binding the protease inhibitor BPTI in solution and confirm this finding. The structural and functional insights of the ZIKV protease presented here should advance our current understanding of flavivirus replication and accelerate structure-based antiviral drug discovery against ZIKV.
ISSN: 2041-1723
DOI: 10.1038/ncomms13410
Schools: School of Biological Sciences 
Lee Kong Chian School of Medicine (LKCMedicine) 
Research Centres: NTU Institute of Structural Biology 
Rights: © 2016 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SBS Journal Articles

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