Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83051
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dc.contributor.authorYap, Karenen
dc.contributor.authorXiao, Yixinen
dc.contributor.authorFriedman, Brad A.en
dc.contributor.authorJe, H. Shawnen
dc.contributor.authorMakeyev, Eugene V.en
dc.date.accessioned2017-05-11T06:00:04Zen
dc.date.accessioned2019-12-06T15:10:53Z-
dc.date.available2017-05-11T06:00:04Zen
dc.date.available2019-12-06T15:10:53Z-
dc.date.issued2016en
dc.identifier.citationYap, K., Xiao, Y., Friedman, B. A., Je, H. S., & Makeyev, E. V. (2016). Polarizing the Neuron through Sustained Co-expression of Alternatively Spliced Isoforms. Cell Reports, 15(6), 1316-1328.en
dc.identifier.issn2211-1247en
dc.identifier.urihttps://hdl.handle.net/10356/83051-
dc.description.abstractAlternative splicing (AS) is an important source of proteome diversity in eukaryotes. However, how this affects protein repertoires at a single-cell level remains an open question. Here, we show that many 30-terminal exons are persistently co-expressed with their alternatives in mammalian neurons. In an important example of this scenario, cell polarity gene Cdc42, a combination of polypyrimidine tract-binding, protein-dependent, and constitutive splicing mechanisms ensures a halfway switch from the general (E7) to the neuron-specific (E6) alternative 30-terminal exon during neuronal differentiation. Perturbing the nearly equimolar E6/E7 ratio in neurons results in defects in both axonal and dendritic compartments and suggests that Cdc42E7 is involved in axonogenesis, whereas Cdc42E6 is required for normal development of dendritic spines. Thus, co-expression of a precise blend of functionally distinct splice isoforms rather than a complete switch from one isoform to another underlies proper structural and functional polarization of neurons.en
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en
dc.format.extent14 p.en
dc.language.isoenen
dc.relation.ispartofseriesCell Reportsen
dc.rights© 2016 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectEukaryotic proteomesen
dc.subjectCdc42 in neuronsen
dc.titlePolarizing the Neuron through Sustained Co-expression of Alternatively Spliced Isoformsen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.1016/j.celrep.2016.04.012en
dc.description.versionPublished versionen
dc.identifier.pmid27134173-
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