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Title: Polarizing the Neuron through Sustained Co-expression of Alternatively Spliced Isoforms
Authors: Yap, Karen
Xiao, Yixin
Friedman, Brad A.
Je, H. Shawn
Makeyev, Eugene V.
Keywords: Eukaryotic proteomes
Cdc42 in neurons
Issue Date: 2016
Source: Yap, K., Xiao, Y., Friedman, B. A., Je, H. S., & Makeyev, E. V. (2016). Polarizing the Neuron through Sustained Co-expression of Alternatively Spliced Isoforms. Cell Reports, 15(6), 1316-1328.
Series/Report no.: Cell Reports
Abstract: Alternative splicing (AS) is an important source of proteome diversity in eukaryotes. However, how this affects protein repertoires at a single-cell level remains an open question. Here, we show that many 30-terminal exons are persistently co-expressed with their alternatives in mammalian neurons. In an important example of this scenario, cell polarity gene Cdc42, a combination of polypyrimidine tract-binding, protein-dependent, and constitutive splicing mechanisms ensures a halfway switch from the general (E7) to the neuron-specific (E6) alternative 30-terminal exon during neuronal differentiation. Perturbing the nearly equimolar E6/E7 ratio in neurons results in defects in both axonal and dendritic compartments and suggests that Cdc42E7 is involved in axonogenesis, whereas Cdc42E6 is required for normal development of dendritic spines. Thus, co-expression of a precise blend of functionally distinct splice isoforms rather than a complete switch from one isoform to another underlies proper structural and functional polarization of neurons.
ISSN: 2211-1247
DOI: 10.1016/j.celrep.2016.04.012
Schools: School of Biological Sciences 
Rights: © 2016 The Authors. This is an open access article under the CC BY license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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