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Title: Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis
Authors: Cui, Fuqiang
Brosché, Mikael
Shapiguzov, Alexey
He, Xin-Qiang
Vainonen, Julia P.
Leppälä, Johanna
Trotta, Andrea
Kangasjärvi, Saijaliisa
Salojärvi, Jarkko
Kangasjärvi, Jaakko
Overmyer, Kirk
Keywords: Chloroplast
Science::Biological sciences
Arabidopsis Thaliana
Issue Date: 2019
Source: Cui, F., Brosché, M., Shapiguzov, A., He, X.-Q., Vainonen, J. P., Leppälä, J., . . . Overmyer, K. (2019). Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis. Free Radical Biology and Medicine, 134, 555-566. doi:10.1016/j.freeradbiomed.2019.02.006
Series/Report no.: Free Radical Biology and Medicine
Abstract: Reactive oxygen species (ROS) are key signalling intermediates in plant metabolism, defence, and stress adaptation. In plants, both the chloroplast and mitochondria are centres of metabolic control and ROS production, which coordinate stress responses in other cell compartments. The herbicide and experimental tool, methyl viologen (MV) induces ROS generation in the chloroplast under illumination, but is also toxic in non-photosynthetic organisms. We used MV to probe plant ROS signalling in compartments other than the chloroplast. Taking a genetic approach in the model plant Arabidopsis (Arabidopsis thaliana), we used natural variation, QTL mapping, and mutant studies with MV in the light, but also under dark conditions, when the chloroplast electron transport is inactive. These studies revealed a light-independent MV-induced ROS-signalling pathway, suggesting mitochondrial involvement. Mitochondrial Mn SUPEROXIDE DISMUTASE was required for ROS-tolerance and the effect of MV was enhanced by exogenous sugar, providing further evidence for the role of mitochondria. Mutant and hormone feeding assays revealed roles for stress hormones in organellar ROS-responses. The radical-induced cell death1 mutant, which is tolerant to MV-induced ROS and exhibits altered mitochondrial signalling, was used to probe interactions between organelles. Our studies suggest that mitochondria are involved in the response to ROS induced by MV in plants.
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.02.006
Schools: School of Biological Sciences 
Rights: © 2019 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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