Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83167
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dc.contributor.authorLi, Jianghuaen
dc.contributor.authorZhang, Kaixien
dc.contributor.authorRuan, Linen
dc.contributor.authorChin, Seow Fongen
dc.contributor.authorWickramasinghe, Nirmanien
dc.contributor.authorLiu, Hanbinen
dc.contributor.authorRavikumar, Vikashinien
dc.contributor.authorRen, Jinghuaen
dc.contributor.authorDuan, Hongweien
dc.contributor.authorYang, Liangen
dc.contributor.authorChan-Park, Mary Bee Engen
dc.date.accessioned2018-07-03T02:03:33Zen
dc.date.accessioned2019-12-06T15:13:09Z-
dc.date.available2018-07-03T02:03:33Zen
dc.date.available2019-12-06T15:13:09Z-
dc.date.issued2018en
dc.identifier.citationLi, J., Zhang, K., Ruan, L., Chin, S. F., Wickramasinghe, N., Liu, H., et al. (2018). Block Copolymer Nanoparticles Remove Biofilms of Drug-Resistant Gram-Positive Bacteria by Nanoscale Bacterial Debridement. Nano Letters.en
dc.identifier.issn1530-6984en
dc.identifier.urihttps://hdl.handle.net/10356/83167-
dc.description.abstractBiofilms and the rapid evolution of multidrug resistance complicate the treatment of bacterial infections. Antibiofilm agents such as metallic–inorganic nanoparticles or peptides act by exerting antibacterial effects and, hence, do not combat biofilms of antibiotics-resistant strains. In this Letter, we show that the block copolymer DA95B5, dextran-block-poly((3-acrylamidopropyl) trimethylammonium chloride (AMPTMA)-co-butyl methacrylate (BMA)), effectively removes preformed biofilms of various clinically relevant multidrug-resistant Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococci (VRE V583), and Enteroccocus faecalis (OG1RF). DA95B5 self-assembles into core–shell nanoparticles with a nonfouling dextran shell and a cationic core. These nanoparticles diffuse into biofilms and attach to bacteria but do not kill them; instead, they promote the gradual dispersal of biofilm bacteria, probably because the solubility of the bacteria–nanoparticle complex is enhanced by the nanoparticle dextran shell. DA95B5, when applied as a solution to a hydrogel pad dressing, shows excellent in vivo MRSA biofilm removal efficacy of 3.6 log reduction in a murine excisional wound model, which is significantly superior to that for vancomycin. Furthermore, DA95B5 has very low in vitro hemolysis and negligible in vivo acute toxicity. This new strategy for biofilm removal (nanoscale bacterial debridement) is orthogonal to conventional rapidly developing resistance traits in bacteria so that it is as effective toward resistant strains as it is toward sensitive strains and may have widespread applications.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en
dc.format.extent46 p.en
dc.language.isoenen
dc.relation.ispartofseriesNano Lettersen
dc.rights© 2018 American Chemical Society (ACS). This is the author created version of a work that has been peer reviewed and accepted for publication by Nano Letters, American Chemical Society (ACS). It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acs.nanolett.8b01000].en
dc.subjectAntibiofilmen
dc.subjectBiocompatibilityen
dc.titleBlock copolymer nanoparticles remove biofilms of drug-resistant gram-positive bacteria by nanoscale bacterial debridementen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en
dc.contributor.researchCentre for Antimicrobial Bioengineeringen
dc.contributor.researchSingapore Centre for Environmental Life Sciences Engineeringen
dc.identifier.doi10.1021/acs.nanolett.8b01000en
dc.description.versionAccepted versionen
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Appears in Collections:LKCMedicine Journal Articles
SCBE Journal Articles
SCELSE Journal Articles
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