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Title: Mesoscopic energy minimization drives pseudomonas aeruginosa biofilm morphologies and consequent stratification of antibiotic activity based on cell metabolism
Authors: Sheraton, Muniraj Vivek
Yam, Joey Kuok Hoong
Tan, Chuan Hao
Oh, H. S.
Mancini, E.
Yang, Liang
Rice, Scott A.
Sloot, Peter M. A.
Keywords: Cellular Potts Model
Mushroom-shaped Biofilm
Issue Date: 2018
Source: Sheraton, M. V., Yam, J. K. H., Tan, C. H., Oh, H. S., Mancini, E., Yang, L. & et al. (2018). Mesoscopic Energy Minimization Drives Pseudomonas aeruginosa Biofilm Morphologies and Consequent Stratification of Antibiotic Activity Based on Cell Metabolism. Antimicrobial Agents and Chemotherapy, 62(5), e02544-17-.
Series/Report no.: Antimicrobial Agents and Chemotherapy
Abstract: Segregation of bacteria based on their metabolic activities in biofilms plays an important role in the development of antibiotic resistance. Mushroom-shaped biofilm structures, which are reported for many bacteria, exhibit topographically varying levels of multiple drug resistance from the cap of the mushroom to its stalk. Understanding the dynamics behind the formation of such structures can aid in design of drug delivery systems, antibiotics, or physical systems for removal of biofilms. We explored the development of metabolically heterogeneous Pseudomonas aeruginosa biofilms using numerical models and laboratory knockout experiments on wild-type and chemotaxis-deficient mutants. We show that chemotactic processes dominate the transformation of slender and hemispherical structures into mushroom structures with a signature cap. Cellular Potts model simulation and experimental data provide evidence that accelerated movement of bacteria along the periphery of the biofilm, due to nutrient cues, results in the formation of mushroom structures and bacterial segregation. Multidrug resistance of bacteria is one of the most threatening dangers to public health. Understanding the mechanisms of the development of mushroom-shaped biofilms helps to identify the multidrug-resistant regions. We decoded the dynamics of the structural evolution of bacterial biofilms and the physics behind the formation of biofilm structures as well as the biological triggers that produce them. Combining in vitro gene knockout experiments with in silico models showed that chemotactic motility is one of the main driving forces for the formation of stalks and caps. Our results provide physicists and biologists with a new perspective on biofilm removal and eradication strategies.
ISSN: 0066-4804
DOI: 10.1128/AAC.02544-17
Schools: School of Materials Science & Engineering 
School of Biological Sciences 
Interdisciplinary Graduate School (IGS) 
Research Centres: Singapore Centre for Environmental Life Sciences Engineering 
Complexity Institute 
Rights: © 2018 Sheraton et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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