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Title: Enhancing the value of nitrogen from rapeseed meal for microbial oil production
Authors: Uçkun Kiran, Esra
Salakkam, Apilak
Trzcinski, Antoine P.
Bakir, Ufuk
Webb, Colin
Keywords: Fermentation
Rapeseed meal
Issue Date: 2012
Source: Uçkun Kiran, E., Salakkam, A., Trzcinski, A. P., Bakir, U., & Webb, C. (2012). Enhancing the value of nitrogen from rapeseed meal for microbial oil production. Enzyme and Microbial Technology, 50(6-7), 337-342.
Series/Report no.: Enzyme and Microbial Technology
Abstract: Rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. Various strategies were tested for the production of a novel fermentation medium, rich in free amino nitrogen (FAN): commercial enzymes (CEs) (2.7 mg g−1 dry meal), liquid state fungal pre-treatment (LSF) using Aspergillus oryzae (4.6 mg g−1), liquid state fungal pre-treatment followed by fungal autolysis (LSFA) (9.13 mg g−1), liquid state pre-treatment using fungal enzymatic broth (EB) (2.1 mg g−1), but the best strategy was a solid state fungal pre-treatment followed by fungal autolysis (34.5 mg g−1). The bioavailability of the nitrogen sources in the novel medium was confirmed in fed-batch bioreactor studies, in which 82.3 g dry cell L−1 of the oleaginous yeast Rhodosporidium toruloides Y4 was obtained with a lipid content of 48%. The dry cell weight obtained was higher than that obtained using conventional yeast extract, due to a higher total nitrogen content in the novel biomedium. The fatty acids obtained from the microbial oil were similar to those derived from rapeseed oil.
ISSN: 0141-0229
DOI: 10.1016/j.enzmictec.2012.03.004
Research Centres: Nanyang Environment and Water Research Institute 
Rights: © 2012 Elsevier Inc. This is the author created version of a work that has been peer reviewed and accepted for publication by Enzyme and Microbial Technology, Elsevier Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:NEWRI Journal Articles

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