Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83403
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dc.contributor.authorChua, Eugene Yue Daoen
dc.contributor.authorVogirala, Vinod Kumaren
dc.contributor.authorInian, Oviyaen
dc.contributor.authorWong, Andrew See Wengen
dc.contributor.authorNordenskiold, Larsen
dc.contributor.authorPlitzko, Juergen M.en
dc.contributor.authorDanev, Radostinen
dc.contributor.authorSandin, Saraen
dc.date.accessioned2016-09-26T02:56:11Zen
dc.date.accessioned2019-12-06T15:21:44Z-
dc.date.available2016-09-26T02:56:11Zen
dc.date.available2019-12-06T15:21:44Z-
dc.date.copyright2016en
dc.date.issued2016en
dc.identifier.citationChua, E. Y. D., Vogirala, V. K., Inian, O., Wong, A. S. W., Nordenskiold, L., Plitzko, J. M., et al. (2016). 3.9 Å structure of the nucleosome core particle determined by phase-plate cryo-EM. Nucleic Acids Research, 44(17), 8013-8019.en
dc.identifier.issn0305-1048en
dc.identifier.urihttps://hdl.handle.net/10356/83403-
dc.description.abstractThe Volta phase plate is a recently developed electron cryo-microscopy (cryo-EM) device that enables contrast enhancement of biological samples. Here we have evaluated the potential of combining phase-plate imaging and single particle analysis to determine the structure of a small protein–DNA complex. To test the method, we made use of a 200 kDa Nucleosome Core Particle (NCP) reconstituted with 601 DNA for which a high-resolution X-ray crystal structure is known. We find that the phase plate provides a significant contrast enhancement that permits individual NCPs and DNA to be clearly identified in amorphous ice. The refined structure from 26,060 particles has an overall resolution of 3.9 Å and the density map exhibits structural features consistent with the estimated resolution, including clear density for amino acid side chains and DNA features such as the phosphate backbone. Our results demonstrate that phase-plate cryo-EM promises to become an important method to determine novel near-atomic resolution structures of small and challenging samples, such as nucleosomes in complex with nucleosome-binding factors.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent7 p.en
dc.language.isoenen
dc.relation.ispartofseriesNucleic Acids Researchen
dc.rights© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.en
dc.subjectNucleosome Core Particleen
dc.subjectcryo-EMen
dc.title3.9 Å structure of the nucleosome core particle determined by phase-plate cryo-EMen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.contributor.researchNTU Institute of Structural Biologyen
dc.identifier.doi10.1093/nar/gkw708en
dc.description.versionPublished versionen
dc.identifier.pmid27563056-
dc.identifier.rims195117en
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