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Title: A novel toolbox to investigate tissue spatial organization applied to the study of the islets of Langerhans
Authors: Tran Thi Nhu, Hoa
Arrojo E. Drigo, Rafael
Berggren, Per-Olof
Boudier, Thomas
Keywords: Image processing
Issue Date: 2017
Source: Tran Thi Nhu, H., Arrojo E. Drigo, R., Berggren, P. O., & Boudier, T. (2017). A novel toolbox to investigate tissue spatial organization applied to the study of the islets of Langerhans. Scientific Reports, 7, 44261-.
Series/Report no.: Scientific Reports
Abstract: Thanks to the development of new 3D Imaging techniques, volumetric data of thick samples, especially tissues, are commonly available. Several algorithms were proposed to analyze cells or nuclei in tissues, however these tools are limited to two dimensions. Within any given tissue, cells are not likely to be organized randomly and as such have specific patterns of cell-cell interaction forming complex communication networks. In this paper, we propose a new set of tools as an approach to segment and analyze tissues in 3D with single cell resolution. This new tool box can identify and compute the geographical location of single cells and analyze the potential physical interactions between different cell types and in 3D. As a proof-of-principle, we applied our methodology to investigation of the cyto-architecture of the islets of Langerhans in mice and monkeys. The results obtained here are a significant improvement in current methodologies and provides new insight into the organization of alpha cells and their cellular interactions within the islet’s cellular framework.
ISSN: 2045-2322
DOI: 10.1038/srep44261
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
Rights: © 2017 The Author(s) (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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