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Title: Nerve regeneration by human corneal stromal keratocytes and stromal fibroblasts
Authors: Yam, Gary Hin-Fai
Williams, Geraint P.
Setiawan, Melina
Yusoff, Nur Zahirah Binte M.
Lee, Xiao-wen
Htoon, Hla Myint
Zhou, Lei
Fuest, Matthias
Mehta, Jodhbir Singh
Keywords: Cell growth
Translational research
Issue Date: 2017
Source: Yam, G. H.-F., Williams, G. P., Setiawan, M., Yusoff, N. Z. B. M., Lee, X.-w., Htoon, H. M., et al. (2017). Nerve regeneration by human corneal stromal keratocytes and stromal fibroblasts. Scientific Reports, 7, 45396-.
Series/Report no.: Scientific Reports
Abstract: Laser refractive surgeries reshape corneal stroma to correct refractive errors, but unavoidably affect corneal nerves. Slow nerve regeneration and atypical neurite morphology cause desensitization and neuro-epitheliopathy. Following injury, surviving corneal stromal keratocytes (CSKs) are activated to stromal fibroblasts (SFs). How these two different cell types influence nerve regeneration is elusive. Our study evaluated the neuro-regulatory effects of human SFs versus CSKs derived from the same corneal stroma using an in vitro chick dorsal root ganglion model. The neurite growth was assessed by a validated concentric circle intersection count method. Serum-free conditioned media (CM) from SFs promoted neurite growth dose-dependently, compared to that from CSKs. We detected neurotrophic and pro-inflammatory factors (interleukin-8, interleukin-15, monocyte chemoattractant protein-1, eotaxin, RANTES) in SFCM by Bio-Plex Human Cytokine assay. More than 130 proteins in SFCM and 49 in CSKCM were identified by nanoLC-MS/MS. Proteins uniquely present in SFCM had reported neuro-regulatory activities and were predicted to regulate neurogenesis, focal adhesion and wound healing. Conclusively, this was the first study showing a physiological relationship between nerve growth and the metabolically active SFs versus quiescent CSKs from the same cornea source. The dose-dependent effect on neurite growth indicated that nerve regeneration could be influenced by SF density.
ISSN: 2045-2322
DOI: 10.1038/srep45396
Schools: School of Materials Science & Engineering 
Rights: © 2017 The Author(s) (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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