Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83591
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dc.contributor.authorGao, Yuen
dc.contributor.authorLim, Jingen
dc.contributor.authorYeo, David Chen Loongen
dc.contributor.authorLiao, Shanshanen
dc.contributor.authorLans, Malinen
dc.contributor.authorWang, Yaqien
dc.contributor.authorTeoh, Swee-Hinen
dc.contributor.authorGoh, Bee Tinen
dc.contributor.authorXu, Chenjieen
dc.date.accessioned2017-06-13T04:06:15Zen
dc.date.accessioned2019-12-06T15:26:17Z-
dc.date.available2017-06-13T04:06:15Zen
dc.date.available2019-12-06T15:26:17Z-
dc.date.issued2016en
dc.identifier.citationGao, Y., Lim, J., Yeo, D. C. L., Liao, S., Lans, M., Wang, Y., et al. (2016). A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles. ACS Applied Materials & Interfaces, 8(10), 6336-6343.en
dc.identifier.issn1944-8244en
dc.identifier.urihttps://hdl.handle.net/10356/83591-
dc.description.abstractCellular labeling with inorganic nanoparticles such as magnetic iron oxide nanoparticles, quantum dots, and fluorescent silica nanoparticles is an important method for the noninvasive visualization of cells using various imaging modalities. Currently, this is mainly achieved through the incubation of cultured cells with the nanoparticles that eventually reach the intracellular compartment through specific or nonspecific internalization. This classic method is advantageous in terms of simplicity and convenience, but it suffers from issues such as difficulties in fully removing free nanoparticles (suspended in solution) and the lack of selectivity on cell types. This article reports an innovative strategy for the specific labeling of adherent cells without the concern of freely suspended nanoparticles. This method relies on a nanocomposite film that is prepared by homogeneously dispersing nanoparticles within a biodegradable polymeric film. When adherent cells are seeded on the film, they adhere, spread, and filtrate into the film through the micropores formed during the film fabrication. The pre-embedded nanoparticles are thus internalized by the cells during this infiltration process. As an example, fluorescent silica nanoparticles were homogeneously distributed within a polycaprolactone film by utilizing cryomilling and heat pressing. Upon incubation within physiological buffer, no silica nanoparticles were released from the nanocomposite film even after 20 d of incubation. However, when adherent cells (e.g., human mesenchymal stem cells) were grown on the film, they became fluorescent after 3 d, which suggests internalization of silica nanoparticles by cells. In comparison, the suspension cells (e.g., monocytes) in the medium remained nonfluorescent no matter whether there was the presence of adherent cells or not. This strategy eventually allowed the selective and concomitant labeling of mesenchymal stem cells during their harvest from bone marrow aspiration.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent21 p.en
dc.language.isoenen
dc.relation.ispartofseriesACS Applied Materials & Interfacesen
dc.rights© 2016 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by ACS Applied Materials & Interfaces, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acsami.5b12409].en
dc.subjectSelective cell labelingen
dc.subjectNanoparticlesen
dc.titleA Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticlesen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.contributor.researchNTU-Northwestern Institute for Nanomedicineen
dc.identifier.doi10.1021/acsami.5b12409en
dc.description.versionAccepted versionen
item.grantfulltextopen-
item.fulltextWith Fulltext-
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