Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83591
Title: A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles
Authors: Gao, Yu
Lim, Jing
Yeo, David Chen Loong
Liao, Shanshan
Lans, Malin
Wang, Yaqi
Teoh, Swee-Hin
Goh, Bee Tin
Xu, Chenjie
Keywords: Selective cell labeling
Nanoparticles
Issue Date: 2016
Source: Gao, Y., Lim, J., Yeo, D. C. L., Liao, S., Lans, M., Wang, Y., et al. (2016). A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles. ACS Applied Materials & Interfaces, 8(10), 6336-6343.
Series/Report no.: ACS Applied Materials & Interfaces
Abstract: Cellular labeling with inorganic nanoparticles such as magnetic iron oxide nanoparticles, quantum dots, and fluorescent silica nanoparticles is an important method for the noninvasive visualization of cells using various imaging modalities. Currently, this is mainly achieved through the incubation of cultured cells with the nanoparticles that eventually reach the intracellular compartment through specific or nonspecific internalization. This classic method is advantageous in terms of simplicity and convenience, but it suffers from issues such as difficulties in fully removing free nanoparticles (suspended in solution) and the lack of selectivity on cell types. This article reports an innovative strategy for the specific labeling of adherent cells without the concern of freely suspended nanoparticles. This method relies on a nanocomposite film that is prepared by homogeneously dispersing nanoparticles within a biodegradable polymeric film. When adherent cells are seeded on the film, they adhere, spread, and filtrate into the film through the micropores formed during the film fabrication. The pre-embedded nanoparticles are thus internalized by the cells during this infiltration process. As an example, fluorescent silica nanoparticles were homogeneously distributed within a polycaprolactone film by utilizing cryomilling and heat pressing. Upon incubation within physiological buffer, no silica nanoparticles were released from the nanocomposite film even after 20 d of incubation. However, when adherent cells (e.g., human mesenchymal stem cells) were grown on the film, they became fluorescent after 3 d, which suggests internalization of silica nanoparticles by cells. In comparison, the suspension cells (e.g., monocytes) in the medium remained nonfluorescent no matter whether there was the presence of adherent cells or not. This strategy eventually allowed the selective and concomitant labeling of mesenchymal stem cells during their harvest from bone marrow aspiration.
URI: https://hdl.handle.net/10356/83591
http://hdl.handle.net/10220/42663
ISSN: 1944-8244
DOI: 10.1021/acsami.5b12409
Rights: © 2016 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by ACS Applied Materials & Interfaces, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acsami.5b12409].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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