Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83788
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dc.contributor.authorHansen, Gorm Mørken
dc.contributor.authorNilsson, Martinen
dc.contributor.authorNielsen, Claus Henriken
dc.contributor.authorHolmstrup, Palleen
dc.contributor.authorHelqvist, Steffenen
dc.contributor.authorTolker-Nielsen, Timen
dc.contributor.authorGivskov, Michaelen
dc.contributor.authorHansen, Peter Riisen
dc.contributor.editorYilmaz, Özlemen
dc.date.accessioned2016-02-15T08:12:55Zen
dc.date.accessioned2019-12-06T15:32:05Z-
dc.date.available2016-02-15T08:12:55Zen
dc.date.available2019-12-06T15:32:05Z-
dc.date.issued2015en
dc.identifier.citationHansen, G. M., Nilsson, M., Nielsen, C. H., Holmstrup, P., Helqvist, S., Tolker-Nielsen, T., et al. (2015). Absence of Bacteria on Coronary Angioplasty Balloons from Unselected Patients: Results with Use of a High Sensitivity Polymerase Chain Reaction Assay. PLOS ONE, 10(12), e0145657-.en
dc.identifier.issn1932-6203en
dc.identifier.urihttps://hdl.handle.net/10356/83788-
dc.description.abstractPeriodontitis is a chronic, bacterially-induced inflammatory disease of the tooth-supporting tissues, which may result in transient bacteremia and a systemic inflammatory response. Periodontitis is associated with coronary artery disease independently of established cardiovascular risk factors, and translocation of bacteria from the oral cavity to the coronary arteries may play a role in the development of coronary artery disease. Very few studies have used angioplasty balloons for in vivo sampling from diseased coronary arteries, and with varying results. Therefore, the aim of this study was to assess if bacterial DNA from primarily oral bacteria could be detected on coronary angioplasty balloons by use of an optimized sampling process combined with an internally validated sensitive polymerase chain reaction (PCR) assay. Coronary angioplasty balloons and control samples from a total of 45 unselected patients with stable angina, unstable angina/non-ST elevation myocardial infarction, and ST-elevation myocardial infarction (n = 15 in each group) were collected and analyzed using a PCR assay with high sensitivity and specificity for 16S rRNA genes of the oral microbiome. Despite elimination of extraction and purification steps, and demonstration of sensitivity levels of 25–125 colony forming units (CFU), we did not detect bacterial DNA from any of the coronary angioplasty balloons. A subsequent questionnaire indicated that the prevalence of periodontitis in the study cohort was at least 39.5%. Although coronary angioplasty balloons are unlikely to be useful for detection of bacteria with current PCR techniques in unselected patients with coronary artery disease, more studies are warranted to determine the extent to which bacteria contribute to atherosclerosis and its clinical manifestations and whether the presence of bacteria in the arteries is a transient phenomenon.en
dc.format.extent11 p.en
dc.language.isoenen
dc.relation.ispartofseriesPLOS ONEen
dc.rights© 2015 Hansen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.subjectcoronary artery diseaseen
dc.subjectbacterial DNAen
dc.titleAbsence of Bacteria on Coronary Angioplasty Balloons from Unselected Patients: Results with Use of a High Sensitivity Polymerase Chain Reaction Assayen
dc.typeJournal Articleen
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen
dc.identifier.doi10.1371/journal.pone.0145657en
dc.description.versionPublished versionen
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item.grantfulltextopen-
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