Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83805
Title: Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus
Authors: Hou, Han Wei
Petchakup, Chayakorn
Tay, Hui Min
Tam, Zhi Yang
Dalan, Rinkoo
Chew, Daniel Ek Kwang
Li, King Ho Holden
Boehm, Bernhard Otto
Keywords: microfluidic neutrophil
diabetes mellitus
Issue Date: 2016
Source: Hou, H. W., Petchakup, C., Tay, H. M., Tam, Z. Y., Dalan, R., Chew, D. E. K., et al. (2016). Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus. Scientific Reports, 6, 29410-.
Series/Report no.: Scientific Reports
Abstract: Advanced management of dysmetabolic syndromes such as diabetes will benefit from a timely mechanistic insight enabling personalized medicine approaches. Herein, we present a rapid microfluidic neutrophil sorting and functional phenotyping strategy for type 2 diabetes mellitus (T2DM) patients using small blood volumes (fingerprick ~100 μL). The developed inertial microfluidics technology enables single-step neutrophil isolation (>90% purity) without immuno-labeling and sorted neutrophils are used to characterize their rolling behavior on E-selectin, a critical step in leukocyte recruitment during inflammation. The integrated microfluidics testing methodology facilitates high throughput single-cell quantification of neutrophil rolling to detect subtle differences in speed distribution. Higher rolling speed was observed in T2DM patients (P < 0.01) which strongly correlated with neutrophil activation, rolling ligand P-selectin glycoprotein ligand 1 (PSGL-1) expression, as well as established cardiovascular risk factors (cholesterol, high-sensitive C-reactive protein (CRP) and HbA1c). Rolling phenotype can be modulated by common disease risk modifiers (metformin and pravastatin). Receiver operating characteristics (ROC) and principal component analysis (PCA) revealed neutrophil rolling as an important functional phenotype in T2DM diagnostics. These results suggest a new point-of-care testing methodology, and neutrophil rolling speed as a functional biomarker for rapid profiling of dysmetabolic subjects in clinical and patient-oriented settings.
URI: https://hdl.handle.net/10356/83805
http://hdl.handle.net/10220/41457
ISSN: 2045-2322
DOI: 10.1038/srep29410
Rights: © 2016 The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
MAE Journal Articles

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