Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83806
Title: Ablating the aryl hydrocarbon receptor (AhR) in CD11c+ cells perturbs intestinal epithelium development and intestinal immunity
Authors: Chng, Song Hui
Kundu, Parag
Dominguez-Brauer, Carmen
Teo, Wei Ling
Mak, Tak Wah
Pettersson, Sven
Kawajiri, Kaname
Fujii-Kuriyama, Yoshiaki
Keywords: Innate immunity
Mucosal immunology
Issue Date: 2016
Source: Chng, S. H., Kundu, P., Dominguez-Brauer, C., Teo, W. L., Kawajiri, K., Fujii-Kuriyama, Y., et al. (2016). Ablating the aryl hydrocarbon receptor (AhR) in CD11c+ cells perturbs intestinal epithelium development and intestinal immunity. Scientific Reports, 6, 23820-.
Series/Report no.: Scientific Reports
Abstract: Diet and microbiome derived indole derivatives are known to activate the ligand induced transcription factor, the Aryl hydrocarbon Receptor (AhR). While the current understanding of AhR biology has confirmed its role in mucosal lymphocytes, its function in intestinal antigen presenting cells (APCs) is poorly understood. Here, we report that Cre-mediated deletion of AhR in CD11c-expressing cells in C57/BL6 mice is associated with altered intestinal epithelial morphogenesis in vivo. Moreover, when co-cultured with AhR-deficient DCs ex vivo, intestinal organoids showed reduced SRY (sex determining region Y)-box 9 and increased Mucin 2 expression, which correlates with reduced Paneth cells and increased goblet cell differentiation, similar to the data obtained in vivo. Further, characterization of intestinal APC subsets, devoid of AhR, revealed an expression pattern associated with aberrant intrinsic Wnt pathway regulation. At a functional level, the loss of AhR in APCs resulted in a dysfunctional epithelial barrier, associated with a more aggressive chemically induced colitis compared to wild type animals. Our results are consistent with a model whereby the AhR signalling pathway may participate in the regulation of innate immunity through intestinal epithelium development and mucosal immunity.
URI: https://hdl.handle.net/10356/83806
http://hdl.handle.net/10220/41461
ISSN: 2045-2322
DOI: 10.1038/srep23820
Rights: © 2016 The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SBS Journal Articles
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