Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83843
Title: High throughput and high yield nanofabrication of precisely designed gold nanohole arrays for fluorescence enhanced detection of biomarkers
Authors: Wong, Ten It
Han, Shan
Wu, Lin
Wang, Yi
Deng, Jie
Tan, Christina Yuan Ling
Bai, Ping
Loke, Yee Chong
Yang, Xin Da
Tse, Man Siu
Ng, Sum Huan
Zhou, Xiaodong
Keywords: gold nanoparticle
fluorescence analysis
Issue Date: 2013
Source: Wong, T. I., Han, S., Wu, L., Wang, Y., Deng, J., Tan, C. Y. L., et al. (2013). High throughput and high yield nanofabrication of precisely designed gold nanohole arrays for fluorescence enhanced detection of biomarkers. Lab on a Chip, 13(12), 2405-2413.
Series/Report no.: Lab on a Chip
Abstract: Fluorescence excitation enhancement by plasmonic nanostructures such as gold nanohole arrays has been a hot topic in biosensing and bioimaging in recent years. However, the high throughput and high yield fabrication of precisely designed metal nanostructures for optimized fluorescence excitation remains a challenge. Our work is the first report combining nanopattern nickel mould fabrication and UV imprinting for gold nanostructure mass fabrication in high yield. We report our successful gold nanohole array mass fabrication on a 4′′ glass wafer, by first fabricating a high fidelity nickel mould, then using the mould for UV nanoimprinting on a polymer coated on the glass, evaporating the gold film on the glass wafer, and lifting off the polymer to obtain a gold nanohole array on the glass. Our optimized process for wafer fabrication can achieve almost 100% yield from nanoimprinting to gold lift-off, while the fabricated nickel mould has >70% defect-free area with the rest having a few scattered defects. In our work, the size and pitch of the gold nanohole array are designed to enhance the fluorescent dye Alexa 647. When the fabricated gold nanohole array is used for prostate specific antigen (PSA) detection by establishing a sandwiched fluorescence assay on the gold surface, a detection limit of 100 pg ml−1 is achieved, while with a same thickness of gold film, only 1 ng ml−1 is detected.
URI: https://hdl.handle.net/10356/83843
http://hdl.handle.net/10220/41482
ISSN: 1473-0197
DOI: 10.1039/c3lc41396a
Rights: © 2013 The Royal Society of Chemistry.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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