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dc.contributor.authorKalailingam, Pazhanichamyen
dc.contributor.authorTan, Kai Weien
dc.contributor.authorTan, Hui Bingen
dc.contributor.authorSng, Ming Keaten
dc.contributor.authorChan, Jeremy Soon Kiaten
dc.contributor.authorTan, Nguan Soonen
dc.contributor.authorThanabalu, Thirumaranen
dc.contributor.authorJain, Neerajen
dc.identifier.citationJain, N., Kalailingam, P., Tan, K. W., Tan, H. B., Sng, M. K., Chan, J. S. K., et al. (2016). Conditional knockout of N-WASP in mouse fibroblast caused keratinocyte hyper proliferation and enhanced wound closure. Scientific Reports, 6, 38109-.en
dc.description.abstractNeural-Wiskott Aldrich Syndrome Protein (N-WASP) is expressed ubiquitously, regulates actin polymerization and is essential during mouse development. We have previously shown that N-WASP is critical for cell-ECM adhesion in fibroblasts. To characterize the role of N-WASP in fibroblast for skin development, we generated a conditional knockout mouse model in which fibroblast N-WASP was ablated using the Cre recombinase driven by Fibroblast Specific Protein promoter (Fsp-Cre). N-WASPFKO (N-WASPfl/fl; Fsp-cre) were born following Mendelian genetics, survived without any visible abnormalities for more than 1 year and were sexually reproductive, suggesting that expression of N-WASP in fibroblast is not critical for survival under laboratory conditions. Histological sections of N-WASPFKO mice skin (13 weeks old) showed thicker epidermis with higher percentage of cells staining for proliferation marker (PCNA), suggesting that N-WASP deficient fibroblasts promote keratinocyte proliferation. N-WASPFKO mice skin had elevated collagen content, elevated expression of FGF7 (keratinocyte growth factor) and TGFβ signaling proteins. Wound healing was faster in N-WASPFKO mice compared to control mice and N-WASP deficient fibroblasts were found to have enhanced collagen gel contraction properties. These results suggest that N-WASP deficiency in fibroblasts improves wound healing by growth factor-mediated enhancement of keratinocyte proliferation and increased wound contraction in mice.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent13 p.en
dc.relation.ispartofseriesScientific Reportsen
dc.rights© 2016 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
dc.subjectSkin modelsen
dc.titleConditional knockout of N-WASP in mouse fibroblast caused keratinocyte hyper proliferation and enhanced wound closureen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.description.versionPublished versionen
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