Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/84169
Title: The effect of linker DNA on the structure and interaction of nucleosome core particles
Authors: Huang, Yen-Chih
Su, Chun-Jen
Korolev, Nikolay
Berezhnoy, Nikolay V.
Wang, Sai
Soman, Aghil
Chen, Chun-Yu
Chen, Hsin-Lung
Jeng, U-Ser
Nordenskiöld, Lars
Keywords: Nucleosome
Science::Biological sciences
DNA
Issue Date: 2018
Source: Huang, Y.-C., Su, C.-J., Korolev, N., Berezhnoy, N. V., Wang, S., Soman, A., . . . Nordenskiöld, L. (2018). The effect of linker DNA on the structure and interaction of nucleosome core particles. Soft Matter, 14(45), 9096-9106. doi:10.1039/C8SM00998H
Series/Report no.: Soft Matter
Abstract: In eukaryotes, the compaction of chromatin fibers composed of nucleosome core particles (NCPs) connected by a linker DNA into chromosomes is highly efficient; however, the underlying folding mechanisms remain elusive. We used small angle X-ray scattering (SAXS) to investigate the influence of linker DNA length on the local structure and the interparticle interactions of the NCPs. In the presence of the linker DNA of 30 bp or less in length, the results suggest partial unwrapping of nucleosomal DNA on the NCP irrespective of the linker DNA length. Moreover, the presence of 15 bp linker DNA alleviated the electrostatic repulsion between the NCPs and prevented the formation of an ordered columnar hexagonal phase, demonstrating that the linker DNA plays an active role in chromatin folding.
URI: https://hdl.handle.net/10356/84169
http://hdl.handle.net/10220/50103
ISSN: 1744-683X
DOI: 10.1039/C8SM00998H
Rights: © 2018 The Royal Society of Chemistry. All rights reserved. This paper was published in Soft Matter and is made available with permission of The Royal Society of Chemistry.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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