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|Title:||Cyclin-Dependent Kinase-Dependent Phosphorylation of Sox2 at Serine 39 Regulates Neurogenesis||Authors:||Lim, Shuhui
Bragado Alonso, Sara
Cheok, Chit Fang
Stanton, Lawrence W.
|Keywords:||Neural stem cells
Cell cycle regulation
|Issue Date:||2017||Source:||Lim, S., Bhinge, A., Bragado Alonso, S., Aksoy, I., Aprea, J., Cheok, C. F., et al. (2017). Cyclin-Dependent Kinase-Dependent Phosphorylation of Sox2 at Serine 39 Regulates Neurogenesis. Molecular and Cellular Biology, 37(16), e00201-17-.||Series/Report no.:||Molecular and Cellular Biology||Abstract:||Sox2 is known to be important for neuron formation, but the precise mechanism through which it activates a neurogenic program and how this differs from its well-established function in self-renewal of stem cells remain elusive. In this study, we identified a highly conserved cyclin-dependent kinase (Cdk) phosphorylation site on serine 39 (S39) in Sox2. In neural stem cells (NSCs), phosphorylation of S39 enhances the ability of Sox2 to negatively regulate neuronal differentiation, while loss of phosphorylation is necessary for chromatin retention of a truncated form of Sox2 generated during neurogenesis. We further demonstrated that nonphosphorylated cleaved Sox2 specifically induces the expression of proneural genes and promotes neurogenic commitment in vivo. Our present study sheds light on how the level of Cdk kinase activity directly regulates Sox2 to tip the balance between self-renewal and differentiation in NSCs.||URI:||https://hdl.handle.net/10356/84199
|ISSN:||0270-7306||DOI:||10.1128/MCB.00201-17||Rights:||© 2017 American Society for Microbiology (ASM). This paper was published in Molecular and Cellular Biology and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology (ASM). The published version is available at: [http://dx.doi.org/10.1128/MCB.00201-17]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||LKCMedicine Journal Articles|
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