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Title: Micro-engineered perfusable 3D vasculatures for cardiovascular diseases
Authors: Menon, Nishanth Venugopal
Tay, Hui Min
Wee, Soon Nan
Li, King Ho Holden
Hou, Han Wei
Keywords: 3D Vasculatures
Cardiovascular Diseases
Issue Date: 2017
Source: Menon, N. V., Tay, H. M., Wee, S. N., Li, K. H. H., & Hou, H. W. (2017). Micro-engineered perfusable 3D vasculatures for cardiovascular diseases. Lab on a Chip.
Series/Report no.: Lab on a Chip
Abstract: Vessel geometries in microengineered in vitro vascular models are important to recapitulate a pathophysiological microenvironment for the study of flow-induced endothelial dysfunction and inflammation in cardiovascular diseases. Herein, we present a simple and novel extracellular matrix (ECM) hydrogel patterning method to create perfusable vascularized microchannels of different geometries based on the concept of capillary burst valve (CBV). No surface modification is necessary and the method is suitable for different ECM types including collagen, matrigel and fibrin. We first created collagen-patterned, endothelialized microchannels to study barrier permeability and neutrophil transendothelial migration, followed by the development of a biomimetic 3D endothelial-smooth muscle cell (EC-SMC) vascular model. We observed a significant decrease in barrier permeability in the co-culture model during inflammation, which indicates the importance of perivascular cells in ECM remodeling. Finally, we engineered collagen-patterned constricted vascular microchannels to mimic stenosis in atherosclerosis. Whole blood was perfused (1-10 dyne cm-2) into the microdevices and distinct platelet and leukocyte adherence patterns were observed due to increased shear stresses at the constriction, and an additional convective flow through the collagen. Taken together, the developed hydrogel patterning technique enables the formation of unique pathophysiological architectures in organ-on-chip microsystems for real-time study of hemodynamics and cellular interactions in cardiovascular diseases.
ISSN: 1473-0197
DOI: 10.1039/C7LC00607A
Rights: © 2017 The Author(s) (Royal Society of Chemistry). This is the author created version of a work that has been peer reviewed and accepted for publication by Lab on a Chip, The Author(s) (Royal Society of Chemistry). It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
MAE Journal Articles
SCBE Journal Articles

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