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Title: Crystal structure of unlinked NS2B-NS3 protease from Zika virus
Authors: Zhang, Zhenzhen
Li, Yan
Loh, Ying Ru
Phoo, Wint Wint
Hung, Alvin W.
Kang, CongBao
Luo, Dahai
Keywords: Crystal Structure
Zika Virus
Issue Date: 2016
Source: Zhang, Z., Li, Y., Loh, Y. R., Phoo, W. W., Hung, A. W., Kang, C., et al. (2016). Crystal structure of unlinked NS2B-NS3 protease from Zika virus. Science, 354(6319), 1597-1600.
Series/Report no.: Science
Abstract: Zika virus (ZIKV) has rapidly emerged as a global public health concern. Viral NS2B-NS3 protease processes viral polyprotein and is essential for the virus replication, making it an attractive antiviral drug target. We report crystal structures at 1.58-angstrom resolution of the unlinked NS2B-NS3 protease from ZIKV as free enzyme and bound to a peptide reversely oriented at the active site. The unlinked NS2B-NS3 protease adopts a closed conformation in which NS2B engages NS3 to form an empty substrate-binding site. A second protease in the same crystal binds to the residues K14K15G16E17 from the neighboring NS3 in reverse orientation, resisting proteolysis. These features of ZIKV NS2B-NS3 protease may accelerate the discovery of structure-based antiviral drugs against ZIKV and related pathogenic flaviviruses.
ISSN: 0036-8075
DOI: 10.1126/science.aai9309
Rights: © 2016 The Author(s). This is the author created version of a work that has been peer reviewed and accepted for publication in Science, published by American Association for the Advancement of Science on behalf of The Author(s). It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document.  The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SBS Journal Articles

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