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Title: Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection
Authors: Zhang, Qingwen
Wu, Lin
Wong, Ten It
Zhang, Jinling
Liu, Xiaohu
Zhou, Xiaodong
Bai, Ping
Liedberg, Bo
Wang, Yi
Keywords: Gold nanohole array
Localized surface plasmon
Issue Date: 2017
Source: Zhang, Q., Wu, L., Wong, T. I., Zhang, J., Liu, X., Zhou, X., et al. (2017). Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection. International Journal of Nanomedicine, 12, 2307-2314.
Series/Report no.: International Journal of Nanomedicine
Abstract: Localized surface plasmon (LSP) has been widely applied for the enhancement of fluorescence emission for biosensing owing to its potential for strong field enhancement. However, due to its small penetration depth, LSP offers limited fluorescence enhancement over a whole sensor chip and, therefore, insufficient sensitivity for the detection of biomolecules, especially large molecules. We demonstrate the simultaneous excitation of LSP and propagating surface plasmon (PSP) on an Au nanohole array under Kretschmann configuration for the detection of prostate-specific antigen with a sandwich immunoassay. The proposed method combines the advantages of high field enhancement by LSP and large surface area probed by PSP field. The simulated results indicated that a maximum enhancement of electric field intensity up to 1,600 times can be achieved under the simultaneous excitation of LSP and PSP modes. The sandwich assay of PSA carried out on gold nanohole array substrate showed a limit of detection of 140 fM supporting coexcitation of LSP and PSP modes. The limit of detection was approximately sevenfold lower than that when only LSP was resonantly excited on the same substrate. The results of this study demonstrate high fluorescence enhancement through the coexcitation of LSP and PSP modes and pave a way for its implementation as a highly sensitive bioassay.
ISSN: 1176-9114
DOI: 10.2147/IJN.S128172
Rights: © 2017 Zhang et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License ( By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (
Fulltext Permission: open
Fulltext Availability: With Fulltext
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