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Title: Interference-free Micro/nanoparticle Cell Engineering by Use of High-Throughput Microfluidic Separation
Authors: Yeo, David Chenloong
Wiraja, Christian
Zhou, Yingying
Tay, Hui Min
Xu, Chenjie
Hou, Han Wei
Keywords: Cell engineering
Dean flow fractionation
Cell separation
Issue Date: 2015
Source: Yeo, D. C., Wiraja, C., Zhou, Y., Tay, H. M., Xu, C., & Hou, H. W. (2015). Interference-free Micro/nanoparticle Cell Engineering by Use of High-Throughput Microfluidic Separation. ACS Applied Materials & Interfaces, 7(37), 20855-20864.
Series/Report no.: ACS Applied Materials & Interfaces
Abstract: Engineering cells with active-ingredient-loaded micro/nanoparticles is becoming increasingly popular for imaging and therapeutic applications. A critical yet inadequately addressed issue during its implementation concerns the significant number of particles that remain unbound following the engineering process, which inadvertently generate signals and impart transformative effects onto neighboring nontarget cells. Here we demonstrate that those unbound micro/nanoparticles remaining in solution can be efficiently separated from the particle-labeled cells by implementing a fast, continuous, and high-throughput Dean flow fractionation (DFF) microfluidic device. As proof-of-concept, we applied the DFF microfluidic device for buffer exchange to sort labeled suspension cells (THP-1) from unbound fluorescent dye and dye-loaded micro/nanoparticles. Compared to conventional centrifugation, the depletion efficiency of free dyes or particles was improved 20-fold and the mislabeling of nontarget bystander cells by free particles was minimized. The microfluidic device was adapted to further accommodate heterogeneous-sized mesenchymal stem cells (MSCs). Complete removal of unbound nanoparticles using DFF led to the usage of engineered MSCs without exerting off-target transformative effects on the functional properties of neighboring endothelial cells. Apart from its effectiveness in removing free particles, this strategy is also efficient and scalable. It could continuously process cell solutions with concentrations up to 107 cells·mL–1 (cell densities commonly encountered during cell therapy) without observable loss of performance. Successful implementation of this technology is expected to pave the way for interference-free clinical application of micro/nanoparticle engineered cells.
ISSN: 1944-8244
DOI: 10.1021/acsami.5b06167
Rights: © 2015 American Chemical Society. This paper was published in ACS Applied Materials and Interfaces and is made available as an electronic reprint (preprint) with permission of American Chemical Society. The published version is available at: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SCBE Journal Articles

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