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Title: Oligo-polyphenylenevinylene conjugated oligoelectrolyte membrane insertion molecules selectively disrupt cell envelopes of gram-positive bacteria
Authors: Hinks, Jamie
Poh, Wee Han
Chu, Justin Jang Hann
Loo, Joachim Say Chye
Bazan, Guillermo C.
Hancock, Lynn E.
Wuertz, Stefan
Keywords: DRNTU::Science::Biological sciences::Microbiology
Issue Date: 2015
Source: Hinks, J., Poh, W. H., Chu, J. J. H., Loo, J. S. C., Bazan, G. C., Hancock, L. E., et al. (2015). Oligo-polyphenylenevinylene conjugated oligoelectrolyte membrane insertion molecules selectively disrupt cell envelopes of gram-positive bacteria. Applied and environmental microbiology, 81(6), 1949-1958.
Series/Report no.: Applied and environmental microbiology
Abstract: The modification of microbial membranes to achieve biotechnological strain improvement with exogenous small molecules such as oligo-polyphenylene (OPV) conjugated oligoelectrolyte (COE) membrane insertion molecules (MIMs) is an emerging biotechnological field. Little is known about the interactions of OPV COEs with their target – the bacterial envelope. We studied the toxicity of three previously reported OPV COES with a selection of Gram-negative and Gram-positive organisms and demonstrated that Gram-positive bacteria are more sensitive to OPV COEs than Gram-negative organisms. Transmission Electron Microscopy demonstrated that these MIMs disrupt microbial membranes and that this occurred to a much greater degree in Gram-positive-organisms. We used a number of mutants to probe the nature of MIM interactions with the microbial envelope but were unable to align the membrane perturbation effect of these compounds to previously reported membrane disruption mechanisms of, for example, cationic antimicrobial peptides. Instead the data support the notion that OPV COEs disrupt microbial membranes through a suspected interaction with diphosphatidylglycerol (DPG), a major component of Gram-positive membranes. The integrity of model membranes containing elevated amounts of DPG was disrupted to a greater extent by MIMs than those prepared from E. coli total lipid extracts alone.
DOI: 10.1128/AEM.03355-14
Rights: © 2015 American Society for Microbiology. This paper was published in Applied and Environmental Microbiology and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology. The paper can be found at the following official DOI: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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