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|Title:||Quantum changes in Helicobacter pylori gene expression accompany host-adaptation||Authors:||Chua, Eng-Guan
Wise, Michael J.
Amoyo, Arlaine A.
Perkins, Timothy T.
Marshall, Barry J.
|Issue Date:||2016||Source:||Chua, E.-G., Wise, M. J., Khosravi, Y., Seow, S.-W., Amoyo, A. A., Pettersson, S., et al. (2017). Quantum changes in Helicobacter pylori gene expression accompany host-adaptation. DNA Research, 24(1), 37-49.||Series/Report no.:||DNA Research||Abstract:||Helicobacter pylori is a highly successful gastric pathogen. High genomic plasticity allows its adaptation to changing host environments. Complete genomes of H. pylori clinical isolate UM032 and its mice-adapted serial derivatives 298 and 299, generated using both PacBio RS and Illumina MiSeq sequencing technologies, were compared to identify novel elements responsible for host-adaptation. The acquisition of a jhp0562-like allele, which encodes for a galactosyltransferase, was identified in the mice-adapted strains. Our analysis implies a new β-1,4-galactosyltransferase role for this enzyme, essential for Ley antigen expression. Intragenomic recombination between babA and babB genes was also observed. Further, we expanded on the list of candidate genes whose expression patterns have been mediated by upstream homopolymer-length alterations to facilitate host adaption. Importantly, greater than four-fold reduction of mRNA levels was demonstrated in five genes. Among the down-regulated genes, three encode for outer membrane proteins, including BabA, BabB and HopD. As expected, a substantial reduction in BabA protein abundance was detected in mice-adapted strains 298 and 299 via Western analysis. Our results suggest that the expression of Ley antigen and reduced outer membrane protein expressions may facilitate H. pylori colonisation of mouse gastric epithelium.||URI:||https://hdl.handle.net/10356/85100
|ISSN:||1340-2838||DOI:||10.1093/dnares/dsw046||Rights:||© 2016 The Author. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please firstname.lastname@example.org||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||LKCMedicine Journal Articles|
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