Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/85341
Full metadata record
DC FieldValueLanguage
dc.contributor.authorRiau, Andri Kartasasmitaen
dc.contributor.authorAung, Thet Tunen
dc.contributor.authorSetiawan, Melinaen
dc.contributor.authorYang, Liangen
dc.contributor.authorYam, Gary Hin Faien
dc.contributor.authorBeuerman, Roger W.en
dc.contributor.authorVenkatraman, Subbu Subramanianen
dc.contributor.authorMehta, Jodhbir Singhen
dc.date.accessioned2019-08-28T01:53:58Zen
dc.date.accessioned2019-12-06T16:01:58Z-
dc.date.available2019-08-28T01:53:58Zen
dc.date.available2019-12-06T16:01:58Z-
dc.date.issued2019en
dc.identifier.citationRiau, A. K., Aung, T. T., Setiawan, M., Yang, L., Yam, G. H. F., Beuerman, R. W., . . . Mehta, S. S. (2019). Surface Immobilization of Nano-Silver on Polymeric Medical Devices to Prevent Bacterial Biofilm Formation. Pathogens, 8(3), 93-. doi:10.3390/pathogens8030093en
dc.identifier.issn2076-0817en
dc.identifier.urihttps://hdl.handle.net/10356/85341-
dc.description.abstractBacterial biofilm on medical devices is difficult to eradicate. Many have capitalized the anti-infective capability of silver ions (Ag+) by incorporating nano-silver (nAg) in a biodegradable coating, which is then laid on polymeric medical devices. However, such coating can be subjected to premature dissolution, particularly in harsh diseased tissue microenvironment, leading to rapid nAg clearance. It stands to reason that impregnating nAg directly onto the device, at the surface, is a more ideal solution. We tested this concept for a corneal prosthesis by immobilizing nAg and nano-hydroxyapatite (nHAp) on poly(methyl methacrylate), and tested its biocompatibility with human stromal cells and antimicrobial performance against biofilm-forming pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. Three different dual-functionalized substrates—high Ag (referred to as 75:25 HAp:Ag); intermediate Ag (95:5 HAp:Ag); and low Ag (99:1 HAp:Ag) were studied. The 75:25 HAp:Ag was effective in inhibiting biofilm formation, but was cytotoxic. The 95:5 HAp:Ag showed the best selectivity among the three substrates; it prevented biofilm formation of both pathogens and had excellent biocompatibility. The coating was also effective in eliminating non-adherent bacteria in the culture media. However, a 28-day incubation in artificial tear fluid revealed a ~40% reduction in Ag+ release, compared to freshly-coated substrates. The reduction affected the inhibition of S. aureus growth, but not the P. aeruginosa. Our findings suggest that Ag+ released from surface-immobilized nAg diminishes over time and becomes less effective in suppressing biofilm formation of Gram-positive bacteria, such as S. aureus. This advocates the coating, more as a protection against perioperative and early postoperative infections, and less as a long-term preventive solution.en
dc.format.extent17 p.en
dc.language.isoenen
dc.relation.ispartofseriesPathogensen
dc.rights© 2019 by the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectEngineering::Materialsen
dc.subjectSilveren
dc.subjectToxicityen
dc.titleSurface immobilization of nano-silver on polymeric medical devices to prevent bacterial biofilm formationen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science & Engineeringen
dc.identifier.doi10.3390/pathogens8030093en
dc.description.versionPublished versionen
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:MSE Journal Articles

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.