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Title: Antimicrobial Activity and Cell Selectivity of Synthetic and Biosynthetic Cationic Polymers
Authors: Venkatesh, Mayandi
Barathi, Veluchamy Amutha
Goh, Eunice Tze Leng
Anggara, Raditya
Fazil, Mobashar Hussain Urf Turabe
Ng, Alice Jie Ying
Harini, Sriram
Aung, Thet Tun
Fox, Stephen John
Liu, Shouping
Yang, Liang
Barkham, Timothy Mark Sebastian
Loh, Xian Jun
Verma, Navin Kumar
Beuerman, Roger W.
Lakshminarayanan, Rajamani
Keywords: Antimicrobial Activity
Cationic Polymers
Issue Date: 2017
Source: Venkatesh, M., Barathi, V. A., Goh, E. T. L., Anggara, R., Fazil, M. H. U. T., Ng, A. J. Y., et al. (2017). Antimicrobial Activity and Cell Selectivity of Synthetic and Biosynthetic Cationic Polymers. Antimicrobial Agents and Chemotherapy, 61(10), e00469-17-.
Series/Report no.: Antimicrobial Agents and Chemotherapy
Abstract: The mammalian and microbial cell selectivity of synthetic and biosynthetic cationic polymers has been investigated. Among the polymers with peptide backbones, polymers containing amino side chains display greater antimicrobial activity than those with guanidine side chains, whereas ethylenimines display superior activity over allylamines. The biosynthetic polymer ε-polylysine (εPL) is noncytotoxic to primary human dermal fibroblasts at concentrations of up to 2,000 μg/ml, suggesting that the presence of an isopeptide backbone has greater cell selectivity than the presence of α-peptide backbones. Both εPL and linear polyethylenimine (LPEI) exhibit bactericidal properties by depolarizing the cytoplasmic membrane and disrupt preformed biofilms. εPL displays broad-spectrum antimicrobial properties against antibiotic-resistant Gram-negative and Gram-positive strains and fungi. εPL elicits rapid bactericidal activity against both Gram-negative and Gram-positive bacteria, and its biocompatibility index is superior to those of cationic antiseptic agents and LPEI. εPL does not interfere with the wound closure of injured rabbit corneas. In a rabbit model of bacterial keratitis, the topical application of εPL (0.3%, wt/vol) decreases the bacterial burden and severity of infections caused by Pseudomonas aeruginosa and Staphylococcus aureus strains. In vivo imaging studies confirm that εPL-treated corneas appeared transparent and nonedematous compared to untreated infected corneas. Taken together, our results highlight the potential of εPL in resolving topical microbial infections.
ISSN: 0066-4804
DOI: 10.1128/AAC.00469-17
Rights: © 2017 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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