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dc.contributor.authorKim, Ahramen
dc.contributor.authorNg, Wei Bengen
dc.contributor.authorBernt, Williamen
dc.contributor.authorCho, Nam-Joonen
dc.identifier.citationKim, A., Ng, W. B., Bernt, W., & Cho, N.-J. (2019). Validation of size estimation of nanoparticle tracking analysis on polydisperse macromolecule assembly. Scientific Reports, 9, 2639-. doi:10.1038/s41598-019-38915-xen
dc.description.abstractAs the physicochemical properties of drug delivery systems are governed not only by the material properties which they are compose of but by their size that they conform, it is crucial to determine the size and distribution of such systems with nanometer-scale precision. The standard technique used to measure the size distribution of nanometer-sized particles in suspension is dynamic light scattering (DLS). Recently, nanoparticle tracking analysis (NTA) has been introduced to measure the diffusion coefficient of particles in a sample to determine their size distribution in relation to DLS results. Because DLS and NTA use identical physical characteristics to determine particle size but differ in the weighting of the distribution, NTA can be a good verification tool for DLS and vice versa. In this study, we evaluated two NTA data analysis methods based on maximum-likelihood estimation, namely finite track length adjustment (FTLA) and an iterative method, on monodisperse polystyrene beads and polydisperse vesicles by comparing the results with DLS. The NTA results from both methods agreed well with the mean size and relative variance values from DLS for monodisperse polystyrene standards. However, for the lipid vesicles prepared in various polydispersity conditions, the iterative method resulted in a better match with DLS than the FTLA method. Further, it was found that it is better to compare the native number-weighted NTA distribution with DLS, rather than its converted distribution weighted by intensity, as the variance of the converted NTA distribution deviates significantly from the DLS results.en
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en
dc.format.extent14 p.en
dc.relation.ispartofseriesScientific Reportsen
dc.rights© 2019 The Author(s) (Nature Publishing Group). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
dc.subjectNanoscale Biophysicsen
dc.titleValidation of size estimation of nanoparticle tracking analysis on polydisperse macromolecule assemblyen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.contributor.schoolSchool of Materials Science and Engineeringen
dc.contributor.organizationCentre for Biomimetic Sensor Scienceen
dc.description.versionPublished versionen
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