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Title: Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions
Authors: Law, Yingyu
Kirkegaard, Rasmus Hansen
Cokro, Angel Anisa
Liu, Xianghui
Arumugam, Krithika
Xie, Chao
Stokholm-Bjerregaard, Mikkel
Drautz-Moses, Daniela Isabel
Nielsen, Per Halkjær
Wuertz, Stefan
Williams, Rohan Benjamin Hugh
Keywords: Waste Water
DRNTU::Engineering::Environmental engineering
Issue Date: 2016
Source: Law, Y., Kirkegaard, R. H., Cokro, A. A., Liu, X., Arumugam, K., Xie, C., . . . Williams, R. B. H. (2016). Integrative microbial community analysis reveals full-scale enhanced biological phosphorus removal under tropical conditions. Scientific Reports, 6, 25719-. doi:10.1038/srep25719
Series/Report no.: Scientific Reports
Abstract: Management of phosphorus discharge from human waste is essential for the control of eutrophication in surface waters. Enhanced biological phosphorus removal (EBPR) is a sustainable, efficient way of removing phosphorus from waste water without employing chemical precipitation, but is assumed unachievable in tropical temperatures due to conditions that favour glycogen accumulating organisms (GAOs) over polyphosphate accumulating organisms (PAOs). Here, we show these assumptions are unfounded by studying comparative community dynamics in a full-scale plant following systematic perturbation of operational conditions, which modified community abundance, function and physicochemical state. A statistically significant increase in the relative abundance of the PAO Accumulibacter was associated with improved EBPR activity. GAO relative abundance also increased, challenging the assumption of competition. An Accumulibacter bin-genome was identified from a whole community metagenomic survey, and comparative analysis against extant Accumulibacter genomes suggests a close relationship to Type II. Analysis of the associated metatranscriptome data revealed that genes encoding proteins involved in the tricarboxylic acid cycle and glycolysis pathways were highly expressed, consistent with metabolic modelling results. Our findings show that tropical EBPR is indeed possible, highlight the translational potential of studying competition dynamics in full-scale waste water communities and carry implications for plant design in tropical regions.
DOI: 10.1038/srep25719
Rights: © 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCELSE Journal Articles


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